Method for treating varicocele or male infertility using anthocyanin extracted from black soybean

an anthocyanin and varicocele technology, applied in the direction of biocide, cardiovascular disorder, drug composition, etc., can solve the problems of inability to develop effective drugs, the association of varicocele with male fertility has not yet been elucidated, and the patient burden is larg

Active Publication Date: 2015-03-17
THE CATHOLIC UNIV OF KOREA IND ACADEMIC COOP FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these surgical procedures could impose a heavy burden on the patients, and thus there is a need to develop effective drug therapy that can be more easily applied.
In this regard, studies of the mechanisms of abnormal spermatogenesis and infertility have suggested a reduction in gonadotropin or testosterone formation, increased scrotal temperature, apoptosis, reactive oxygen species, and ischemic changes caused by hypoxia as possible mechanisms inducing injury to the testis, but association of varicocele with male fertility has not yet been elucidated.
Therefore, there are difficulties in developing effective drugs for varicocele or male infertility caused thereby.
However, there are no reports about medical effects of anthocyanin extracted from black soybean on varicocele or male infertility in the prior publications including the above publications.

Method used

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  • Method for treating varicocele or male infertility using anthocyanin extracted from black soybean
  • Method for treating varicocele or male infertility using anthocyanin extracted from black soybean

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Effect test

example 1

Preparation of Materials

[0046]1-1. Extraction and Ingredient Analysis of Anthocyanin

[0047]The black soybean used in the present experiments was supplied by the Rural Development Administration, and its extraction and ingredient analysis were performed by the same methods used in the previous study of the present inventors (Jang H. et al., J Agric Food Chem 58:12686-91, 2010). The seed coats of black soybean (Chungja 3) were peeled manually, and 500 g thereof were extracted using 2000 ml of 80% ethanol (0.1% acetic acid) at 4° C. for 2 days twice. The solution extract was filtered through a 0.45 um filter, and concentrated using a rotary vacuum evaporator (35° C.) to obtain a crude anthocyanin extract. The residual solvent was removed from the crude extract by freeze-drying, and the resulting red powder was stored at −70°.

[0048]HPLC (High performance liquid chromatography) was performed using a Dionex Ultimate 3000 series (Dionex Softron GmbH, Germering, Germany) to analyze the ingre...

example 2

Collection of Testis Tissues and Measurement of Spermatogenic Cell Density

[0056]After 4 weeks, animals were anesthetized, both testes were resected by lower abdominal resection, and the testis weight was measured. Tissues were then obtained, fixed in 10% neutral formalin, embedded in paraffin, stained with Hematoxylin-Eosin, and examined under an optical microscope. Under an optical microscope at 400× magnification, 10 sites of seminiferous tubules that were almost round were selected randomly, and the spermatogenic cell density was obtained by measuring the thickness of the germinal cell layer and the diameter of the seminiferous tubules.

example 3

Collection and Evaluation of Cauda Epididymal Spermatozoa

[0057]Cauda epididymides were minced in 5 ml of normal saline containing 0.5% bovine serum albumin at 37° C. and were then filtered. Sperm suspensions were placed on glass slides that had been pre-warmed at 37° C. Percentages of motile sperm were determined by counting more than 200 spermatozoa in randomly selected fields under an optical microscope. Sperm counts were expressed as the number of motile spermatozoa per gram of cauda epididymis tissue (Kim, S. J., et al., Korean J Urol 33, 404-417, 1992). Samples were evaluated by one expert investigator unaware of sample identities.

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Abstract

The present invention relates to the therapeutic or ameliorating effects of anthocyanin extracted from black soybean on varicocele or male infertility. In particular, the present invention relates to a method for treating or ameliorating varicocele or male infertility using anthocyanin extracted from black soybean.

Description

CROSS REFERENCE TO RELATED APPLICATION[0001]This application claims priority to and the benefit of Korean Patent Application No. 10-2011-0099358 filed on Sep. 29, 2011, which is hereby incorporated by reference for all purposes as if fully set forth herein.BACKGROUND OF THE INVENTION[0002](a) Field of the Invention[0003]The present invention relates to the therapeutic or ameliorating effects of anthocyanin extracted from black soybean on varicocele or male infertility. In particular, the present invention relates to a method for treating or ameliorating varicocele or male infertility using anthocyanin extracted from black soybean.[0004](b) Description of the Related Art[0005]Varicocele is the stretching and twisting of the pampiniform plexus of the spermatic cord through which venous blood flows in from the testis, and it is known to be the most common disease underlying male infertility. About 98% of varicocele occur on the left side, because the longer left testicular vein connect...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): A61K31/7048A61K36/48A23L1/30
CPCA61K31/7048A61K36/48A23L1/3002A23L33/105A61P15/00A61P15/08A61P9/00A61K31/352
Inventor KIM, SAE-WOONGCHUN, MYUNG-HOONKIM, IN-BEOMHA, U-SYNKIM, SU-JINJANG, HOON
Owner THE CATHOLIC UNIV OF KOREA IND ACADEMIC COOP FOUND
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