The invention discloses enzyme soap. The enzyme soap is prepared from, by weight, 10-12 parts of glycerinum, 12-14 parts of glycerinum, 6-7 parts of propylene glycol, 12-13 parts of lauryl polyoxyethylene ether sodium sulfate, 12-14 parts of octadecanoic acid, 2-3 parts of tetradecanoic acid, 8-10 parts of dodecanoic acid, 3-4 parts of NaOH, 1-3 parts of EDTA, 15-18 parts of sorbitol, 7-9 parts of purified water and 4-6 parts of enzymes. The invention further discloses a preparation method of the enzyme soap. The method includes the steps of putting glycerinum, polyvinyl alcohol, propylene glycol and lauryl polyoxyethylene ether sodium sulfate in a stirring tank to be heated, conducting heat preservation at a set temperature, putting octadecanoic acid, tetradecanoic acid and dodecanoic acid in the stirring tank to be heated, conducting heat preservation at a set temperature, mixing NaOH and EDTA with purified water, adding the mixture to the stirring tank to be heated, conducting heat preservation at a set temperature, adding sorbitol to the stirring tank, conducting stirring at a set temperature, adding enzymes to the stirring tank, and conducting filling after uniform stirring. The components in the formula are small in stimulation to skin and have good cleaning, moisturizing and whitening effects, and the preparation process is easy and convenient.