The embodiment of the present invention discloses a pixel structure and its manufacturing method, and a display panel. The pixel structure includes: a substrate, an anode layer, a first auxiliary light-emitting layer, a light-emitting layer, a cathode layer, and at least one first resistance structure, wherein the light-emitting The layer at least includes a first light-emitting portion and a second light-emitting portion, the first light-emitting portion corresponds to the first sub-pixel, the second light-emitting portion corresponds to the second sub-pixel, and the turn-on voltage of the first sub-pixel is greater than the turn-on voltage of the second sub-pixel Voltage, the first resistance structure is located on the path from the first auxiliary light-emitting layer to the cathode layer and in the vertical direction of the second light-emitting part, at least partially overlaps with the second light-emitting part, does not overlap with the first light-emitting part, and at least one The absolute value of the difference between the resistance value of the first resistance structure and the second light-emitting part and the resistance value of the first light-emitting part is smaller than the first preset value, so as to reduce the color of the display panel including the pixel structure. The crosstalk phenomenon improves the display quality of the display panel.