Bioreactor system for nerve stem cell to amplify in large scale and long time
A bioreactor and neural stem cell technology, applied in bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, biochemical equipment, etc., can solve problems such as bacterial contamination, medium supply, lack of flexibility in design, etc. , to achieve the effects of reduced cell loss, reliable test data, and great flexibility
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[0022] Such as Figure 1-5 As shown, the present invention includes: an air supply unit 1, a culture solution supply unit 2, a cell expansion unit 3 and a first peristaltic pump 4, and its connection mode is: the end of the air supply unit 1 is connected to the cell expansion unit 3 through a silica gel air tube The end of the culture solution supply unit 2 is connected to the cell expansion unit 3 through a silicone infusion tube, and the first peristaltic pump 4 is in the middle of the silicone infusion tube.
[0023] The gas supply unit 1 includes: CO 2 Gas cylinder 5, air compressor 6, first gas flow meter 7, second gas flow meter 8, first 0.2 μm microporous membrane filter 9, second 0.2 μm microporous membrane filter 10, gas humidification Device 11, gas humidification device water bath layer 14, second peristaltic pump 17, first water bath 18, third 0.2 μm microporous membrane filter 19 and T-shaped tee pipe 20, CO 2 The gas cylinder 5 is connected to the first gas flo...
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