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Photon crystal composite encoding microsphere and preparation method

A technology of photonic crystal microspheres and photonic crystals, which is applied in the direction of material analysis, measuring devices, instruments, etc. by optical means, can solve the problems of indistinguishable emission peaks of emitted light, complicated detection equipment, complicated preparation, etc., and achieves adjustable color. , the effect of wide application range and simple preparation method

Inactive Publication Date: 2009-12-02
SOUTHEAST UNIV
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AI Technical Summary

Problems solved by technology

The preparation of these encoding methods and encoding carriers is relatively complicated, and the detection equipment required for the decoding process is complex and costly
In addition, the use of fluorescent dyes for coding also has problems such as photofading, difficulty in distinguishing the emission peaks of emitted light, and biocompatibility.

Method used

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  • Photon crystal composite encoding microsphere and preparation method
  • Photon crystal composite encoding microsphere and preparation method

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] The preparation method includes the following steps:

[0026] Select photonic crystal microspheres with different codes and perform surface treatment on them to obtain negatively charged photonic crystal coded microspheres;

[0027] Using the layer-by-layer adsorption method, the positive polyelectrolyte is first adsorbed on the surface of the photonic crystal microsphere, and then the negative polyelectrolyte is adsorbed, and then after the positive polyelectrolyte is adsorbed, the quantum dots with different characteristic emission peaks are adsorbed on the outer surface of the microsphere;

[0028] To absorb quantum dots of different layers or different sizes, the layers are separated by three layers of polyelectrolyte;

[0029] The quantum dots in the outermost layer continue to adsorb four layers of polyelectrolyte to facilitate detection.

[0030] At the same time, use quantum dots of the same kind but different sizes to label the object to be detected, and the concen...

Embodiment 1

[0031] Example one. Preparation of water-soluble CdTe labeled polystyrene microspheres

[0032] 1 Select the polystyrene microspheres coded at 600nm and 540nm respectively. Since the synthesized polystyrene particles have carboxyl groups, the surface of the prepared microspheres is also negatively charged.

[0033] 2 Soak the prepared microspheres in a 0.5mol / L NaCl solution of 1mg / mL PAH, stand for 20 minutes to absorb, rinse with ultrapure water three times to wash off excess PAH. Then the microspheres were immersed in 0.5mol / L NaCl solution of 1mg / mLPSS, and the same was allowed to stand for adsorption and centrifugal washing. After adsorbing another layer of PAH, soak the microspheres in CdTe modified with thioglycolic acid 540 (Emission spectrum at 540nm) in an aqueous solution, stand and absorb for 60 minutes, then centrifuge and wash.

[0034] 3 Repeat the above steps. After adsorbing three layers of polyelectrolyte, then adsorb the second layer of CdTe 540 . By analogy, thre...

Embodiment 2

[0036] 实施例二。 Example two. Preparation of water-soluble CdTe labeled silica microspheres

[0037] 1 Select 400nm, 460nm and 540nm coded silica microspheres respectively, and immerse them in 70% (V / V 0 ) Sulfuric acid and 30% hydrogen peroxide solution overnight, so that the surface of the prepared microspheres is negatively charged.

[0038] 2 Soak the prepared microspheres in a 0.5mol / L NaCl solution of 1mg / mL PAH, stand for 20 minutes to absorb, rinse with ultrapure water three times to wash off excess PAH. Then the microspheres were immersed in 0.5mol / L NaCl solution of 1mg / mLPSS, and the same was allowed to stand for adsorption and centrifugal washing. After adsorbing another layer of PAH, soak the microspheres in CdTe modified with thioglycolic acid 540 (Emission spectrum at 540nm) in an aqueous solution, stand and absorb for 60 minutes and then centrifuge and wash.

[0039] 3 Repeat the above steps. After adsorbing three layers of polyelectrolyte, then adsorb the second layer...

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Abstract

The photonic crystal composite coded microsphere and its preparation method relate to a method in which the microsphere is coded by combining the specific light reflection peak of the photonic crystal and the characteristic emission peak and characteristic emission peak intensity of the quantum dots adsorbed on the outer layer, which is characterized in that the microsphere is composed of colloidal particles After assembling into microspheres, the quantum dots are wrapped on the surface of photonic crystal microspheres by layer-by-layer assembly. By wrapping different quantum dots and different quantum dot layers, the quantum dot code is combined with the photonic crystal microsphere code. The amount of coding is greatly increased. The encoding used in the polymer microsphere has the advantages of good stability, easy control, low manufacturing cost, simplicity and high efficiency, large detection throughput and the like.

Description

Technical field [0001] The invention relates to a method in which a microsphere is compound-encoded by a photonic crystal specific light reflection peak, a quantum dot characteristic emission peak and characteristic emission peak intensity absorbed by an outer layer, and belongs to the technical field of biomedical research, environmental monitoring and clinical detection. Background technique [0002] After the sequence of the human genome is decoded, the function of genes and the interaction between the proteins encoded by the genes have become research hotspots, and humans have entered the post-genome era. A significant feature of the post-genomic era is to study a large number of interactions between proteins and nucleic acids, between proteins and proteins, and between proteins and drugs. The study of these interactions is almost impossible using traditional test methods. Therefore, a high-throughput test platform is needed. High-throughput test platforms require high-throug...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/00
Inventor 顾忠泽李娟赵祥伟赵远锦扈靖
Owner SOUTHEAST UNIV
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