Technique for fabricating label type biologic slice
A production process and slicing technology, which is applied in the field of label-type biological slice production technology, can solve the problems of low production efficiency, poisoning, affecting the consistency of dyeing and sealing of sliced specimens, etc., so as to improve production efficiency and reduce environmental and human pollution. and hazards, the effects of product novelty
Inactive Publication Date: 2009-12-09
段增强
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Problems solved by technology
However, the existing biological slice production process is limited by the carrier, and the sliced specimens cannot be processed centrally. Each slide can only hold one to several sliced specimens. Due to the limitation of the slides, this affects the sliced specimens to a certain extent. Consistency of staining and sealing, and more importantly, only one specimen can be produced at a time, resulting in low production efficiency
Thirdly, the producers have been poisoned by xylene and other harmful drugs for a long time, and it has not been solved so far.
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0011] The Existing Biological Slicing Technology and Its Deficiencies (Taking Paraffin Section as an Example)
[0012] (1) The main steps of the existing bioslicing technology
[0013] Material collection: It depends on the purpose of production, but whether it is animal or plant organ materials, the principle of "small but precise" should be followed when collecting materials to facilitate fast fixation.
[0014] Fixation: Quickly kill and fix the collected materials with formaldehyde, glacial acetic acid, and ethanol liquid to keep them in their original living state.
[0015] Washing with water: After the material is killed and fixed, wash away the fixative in the material with water or 50% ethanol washing solution.
[0016] Dehydration: Use one of ethanol, n-butanol, tert-butanol, and acetone to replace all the moisture in the material.
[0017] Transparent: After the material is dehydrated, xylene is often used as a transparent agent, because it can be mixed with both ...
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Abstract
The invention discloses a label-type biological slicing manufacturing process. The steps from material collection, fixing to displaying are the same as the existing biological slicing technology; and the following unique methods are adopted in the steps from transferring to sealing, (10) Slices: Use epoxy resin material as the slice carrier, place the unfolded slices one by one, each carrier can hold one hundred to hundreds of slices; (11) Dewaxing: After the slices are placed and dried, the Concentrated dewaxing with xylene in a container; (12) Staining: After the wax on the section is completely dissolved by xylene, treat it with absolute ethanol and ethanol with a concentration gradient of 95% to 50%, and then carry out intensive staining. (13) Transparent: After the sections are stained, they are treated with ethanol with a concentration gradient from 50% to absolute ethanol, and then concentrated and transparent with xylene; (14) Mounting: The sections transparent through xylene are sprayed with high-gloss resin cover film. Then it is dried, divided, and according to needs, it is pasted on the middle of the glass slide like a label, so that one hundred to hundreds of slices can be made at a time.
Description
technical field [0001] The invention relates to a biological slicing technology, in particular to a label-type biological slicing manufacturing process. Background technique [0002] The traditional bioslicing technology was introduced into my country in the early 20th century, and has played an important role in the fields of biological research and teaching for a long time. my country has successively established many biological slice specimen factories, providing a large number of biological, medical and other sliced specimens for agricultural, medical, and biological colleges and universities and primary and secondary schools across the country. However, the existing biological slice production process is limited by the carrier, and the sliced specimens cannot be processed centrally. Each slide can only hold one to several sliced specimens. Due to the limitation of the slides, this affects the sliced specimens to a certain extent. Consistency of staining and sea...
Claims
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IPC IPC(8): G01N1/28G09B23/00
Inventor 段增强高致明贺德先田朝阳
Owner 段增强