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Intravascular adult stem cell rack for preventing and treating angiostenosis and preparation method thereof

A technology of adult stem cells and somatic stem cells, applied in the field of intravascular stents coated with adult stem cells, can solve the problems of aggravating restenosis, delaying restenosis, thrombosis restenosis, etc., to avoid inflammation, reduce damage, and reduce intravascular restenosis. narrow effect

Inactive Publication Date: 2010-01-13
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, drug-eluting stents have disadvantages and deficiencies: (1) drug-eluting stents only delay the occurrence of restenosis, rather than inhibit restenosis; (2) polymers used to load drugs can induce chronic inflammation, damage repair and re-endothelialization delay and other adverse reactions; (3) limitations of drugs, the current drugs coated on the stent, while inhibiting the proliferation of vascular smooth muscle cells, also inhibit the proliferation of vascular endothelial cells, triggering the formation of thrombus and stent The occurrence of restenosis in the middle and late stages after implantation
[0005] The above research results show that although the endothelial coverage of the stent is accelerated and the formation of thrombus in the stent is reduced, the anti-restenosis effect has not reached the original assumption. The possible reasons include: (1) The differentiation characteristics of EPC have not been fully clarified , CD34+ EPCs may not have optimal endothelial differentiation potential, and may even differentiate into smooth muscle cells and aggravate restenosis
(2) The specificity of CD34+ antibody capture still needs to be improved. In addition to EPCs, there are also mature endothelial cells and even inflammatory cells

Method used

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  • Intravascular adult stem cell rack for preventing and treating angiostenosis and preparation method thereof
  • Intravascular adult stem cell rack for preventing and treating angiostenosis and preparation method thereof
  • Intravascular adult stem cell rack for preventing and treating angiostenosis and preparation method thereof

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preparation example Construction

[0045] The preparation method of the above scaffold can be to first prepare a protein solution that promotes the adhesion, proliferation and directional differentiation of somatic stem cells into endothelial cells; then, soak the sterilized bare metal scaffold in protein solution A, or spray the protein solution with coated on the outer surface of the bare metal stent to make a protein-coated intravascular stent; the protein solution is prepared as follows: the protein solution includes antibodies and cytokines, and the antibodies and cytokines are respectively After mixing with PBS buffer, then mixing together to make a solution, or mixing antibodies and cytokines together with PBS buffer to make a solution, which is a protein solution; wherein, the antibody is composed of CD133 antibody, CD34 One, or two, or all of antibodies and CD14 antibodies; the cytokines are composed of one, or two, or all of VEGF factors, bFGF factors, and TGF factors; the final concentrations of antib...

Embodiment 1

[0056] (1) Select material 316L stainless steel bracket, ultrasonically clean it in ethanol, acetone, distilled water for 5 minutes, then dry it in a vacuum oven for 60 minutes at a drying temperature of 45 degrees, and finally irradiate it with an ultraviolet lamp for 30 minutes for disinfection;

[0057] (2) Prepare the coating material, the polymer matrix material is polylactic acid and polyvinyl fluoride (1: 1), the solvent is acetone, and the concentration of the polymer in its solvent is 2 mg / ml, at 25 degrees room temperature, using magnetic force Stir with the mixer for 30 minutes, mix well;

[0058] (3) Prepare the polymer coating by ultrasonic atomization spraying, add the prepared polymer matrix material solution into the solution bottle of the spraying device, and form a uniform drug coating on the surface of the stent through the spraying device, the spraying time is 1 minute, in the absence of Operated in the bacterial chamber, the final coating thickness is 20μm...

Embodiment 2

[0063] (1) Select the NiTi alloy bracket as the material, ultrasonically clean it in ethanol, acetone, and distilled water for 5 minutes, then dry it in a vacuum oven for 60 minutes at a drying temperature of 45 degrees, and finally irradiate it with an ultraviolet lamp for 30 minutes for disinfection;

[0064] (2) Prepare the coating material, the polymer matrix material is high-density polyethylene and gelatin (1: 1), the solvent is acetone, and the concentration of the polymer in its solvent is 3 mg / ml, at a temperature of 30 degrees, using magnetic force Stir with the mixer for 30 minutes, mix well;

[0065] (3) Putting the bare stent into the polymer coating solution prepared in step (2), soaking for 10 minutes, drying, and repeating this three times to form a polymer coating with a thickness of 30 μm;

[0066] (4) Mix CD34 antibody, statins and TGF factor three drug solutions (2:1:1), put the stent prepared in step (4) into the mixed solution (3-5ml), soak for 10min, and...

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Abstract

The intravascular adult stem cell rack for preventing and treating angiostenosis includes one bare metal rack and one protein coating coated to the surface of the bare metal rack to promote the adhesion, proliferation and directional differentiation into endothelial cell of the adult stem cell. There may be also one polymer coating in between. The protein coating includes one antibody layer, one polypeptide / medicine layer and one cell factor layer. The present invention can form endothelium in the surface of the rack, decrease injury to endothelium, and prevent and treat angiostenosis.

Description

(1) Technical field [0001] The invention relates to a cell-coated intravascular stent, in particular to an adult stem cell-coated intravascular stent. (two), background technology [0002] Vascular stent is an important carrier for minimally invasive surgery to treat vascular stenosis, and its short-term clinical efficacy after implantation has been widely recognized. Percutaneous transluminal angioplasty (PTA) based on this technology has been developed since the 1980s. It has become a routine therapy that keeps pace with drugs and other surgical operations for the treatment of coronary heart disease. Unfortunately, the long-term placement of bare metal stents has not completely "cured" restenosis, but also led to the emergence of a new problem - In-stent restenosis (ISR). According to clinical statistics, the incidence of vascular restenosis after stent placement is 15-20%, while the incidence of in-stent restenosis is as high as 30-50% in patients with diabetes mellitus,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/16A61L31/12A61F2/82
Inventor 王贵学肖丽
Owner CHONGQING UNIV
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