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Plants having improved growth characteristics and method for making the same

A plant and characteristic technology, applied in the field of molecular biology, to achieve the effect of increasing yield and improving growth characteristics

Inactive Publication Date: 2007-08-08
CROPDESIGN NV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such breeding techniques have several disadvantages, namely that these techniques are often labor-intensive and produce plants that often contain heterologous genetic components that do not always produce the desired character

Method used

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  • Plants having improved growth characteristics and method for making the same
  • Plants having improved growth characteristics and method for making the same
  • Plants having improved growth characteristics and method for making the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0127] Example 1: Gene Cloning

[0128] The Arabidopsis YIPPEE-like gene (CDS1522) was amplified by PCR using the Arabidopsis seedling cDNA library (Invitrogen, Paisley, UK) as a template. After the RNA extracted from the seedlings was reverse transcribed, the cDNA was cloned into pCMV Sport6.0. The library has an average insert size of 1.5kb, and the number of original clones is on the order of 1.59×10 7 cfu. in 6×10 11 After the first amplification of cfu / ml, the original titer was determined to be 9.6 × 10 5 cfu / ml. After plasmid extraction, 200 ng template was used in 50 μl PCR mix. The primers used for PCR amplification (which includes the AttB site of Gateway recombination) are prm03196 (sense, the start codon is indicated in bold, and the AttB1 site is indicated in italics: 5'-GGGGACAAGTTTGTACAAAAAAAGCAGGCTTCACAATGGCTGTCGGAGATGAT-3') and prm03199 (antisense, Complementary, stop codon in bold, AttB2 site in italics: 5'GGGGACCACTTTGTACAAGAAAGCTGGGTAATCAAGCATCATTCCAT...

Embodiment 2

[0129] Embodiment 2: vector construction

[0130] Next, the entry clone p3956 was used in the LR reaction together with the designated vector p0640 for rice transformation. This vector contains the following as functional elements within the T-DNA borders: plant selectable marker; selectable marker expression cassette; Gateway expression cassette for LR intended for in vivo recombination with the sequence of interest cloned into the entry clone . The rice GOS2 promoter (PRO0129) for constitutive expression was upstream of this Gateway cassette (De Pater et al., Plant J. 1992 Nov;2(6):837-44).

Embodiment 3

[0132] Example 3: Evaluation and Results

[0133]Approximately 15 to 20 independent TO rice transformants were generated. Primary transformants were transferred from the tissue culture room to the greenhouse for growth and T1 seeds were harvested. Five events were retained in which the T1 progeny segregated 3:1 for the presence / absence of the transgene. Approximately 10 T1 seedlings containing the transgene (heterozygous and homozygous) and approximately 10 T1 seedlings lacking the transgene (nullzygote) were selected in each event by monitoring the expression of the visible markers.

[0134] Statistical analysis: F-test

[0135] A two-way ANOVA (Analysis of Variation) was used as a statistical model for the overall assessment of plant phenotypic properties. F-tests were performed on all measured parameters in all plants of all events transformed with the gene of the invention. The F-test is performed to check the validity of the gene across all transformation events and t...

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Abstract

The present invention concerns a method for improving the growth characteristics of plants by increasing activity in a plant of a YIPPEE-like polypeptide or a homologue thereof. One such method comprises introducing into a plant a YIPPEE-like nucleic acid or variant thereof. The invention also relates to transgenic plants having introduced therein a YIPPEE-like nucleic acid or variant thereof, which plants have improved growth characteristics relative to corresponding wild type plants. The present invention also concerns constructs useful in the methods of the invention.

Description

technical field [0001] The present invention relates generally to the field of molecular biology, and to methods of improving plant growth characteristics. More specifically, the present invention relates to methods for improving plant growth characteristics, especially yield, by increasing the activity of YIPPEE-like polypeptides or homologues thereof in plants. The invention also relates to plants having increased activity of a YIPPEE-like polypeptide or a homologue thereof, which plants have improved growth characteristics relative to corresponding wild-type plants. The invention also provides constructs useful in the methods of the invention. technical background [0002] The world's growing population and diminishing supply of arable land for agriculture has driven agricultural research toward improving agricultural efficiency. A conventional approach to crop and horticultural improvement is the use of selective breeding techniques to identify plants with desired trai...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/82
CPCC07K14/415C12N15/8261
Inventor C·鲁兹
Owner CROPDESIGN NV
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