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Integrated two-dimensional gel electrophoresis

A technique of gel electrophoresis and gel, applied in the field of electrophoresis components or disposable devices, improved disposable devices

Inactive Publication Date: 2007-08-15
F HOFFMANN LA ROCHE & CO AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, agarose introduces new complications, such as the need to boil it before melting, and the difficulty of removing it from all tubes and apparatus once it begins to gel

Method used

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  • Integrated two-dimensional gel electrophoresis
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Experimental program
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Embodiment Construction

[0028] The steps of the method are performed automatically, as described below with reference to the accompanying drawings:

[0029] 1. Insert the first gel strip 7 or assemble the foil 5 with the strip 7 into the disposable 1 unless the strip is already integrated in the closed disposable 1 . A possible alternative is to cast the IEF strip 7 in situ with the two valves 9 and 10 arranged at both ends closed. The valves are actuated by inserts 12 and 14 which are part of the instrumentation (not shown) used to operate the disposable.

[0030] 2. Load the sample in the rehydration solution, the two valves 9 and 10 on both sides of the strip 7 are closed, the distance between the foil 5 and the soft part valve material 11 covering the inner surface of the main body 3 of the disposable is e.g. 0.7 mm until the bead chamber thus produced is filled.

[0031] 3. Set the temperature of the cooling module to eg 30-35°C and wait for at least one hour for rehydration.

[0032] 4. Run ...

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Abstract

An electrophoresis assembly or disposable device for the separation of a complex protein sample, using two dimensional gel electrophoresis comprises a carrier such as a film or foil or glass plate (5) with an area for the first dimension gel strip (7) and an area (16) for the second dimension gel, where said two areas are directly in contact with each other. The assembly or device further comprises a two component main body made of a hard component (3) and a thin and flexible soft component layer (11) at a variable distance from the carrier (5) which body further comprises at least one slit or opening for external actuation of at least one valve (9, 10) where said valve is represented by said soft component material in correspondence of said at least one slit.

Description

technical field [0001] The present invention relates to electrophoresis assemblies or disposable devices for separating complex protein samples using two-dimensional gel electrophoresis, and methods for separating complex protein samples using two-dimensional gel electrophoresis. [0002] The present invention relates to improved disposable devices and methods of processing said devices; providing a facile and efficient integration of generally accepted manually performed steps for the separation of complex protein samples in two-dimensional gel electrophoresis-based proteome analysis , thereby complementing and extending the scope of the previous EP application 1712903, which also introduced a new method. Background technique [0003] Two-dimensional slab gel electrophoresis remains the most commonly used method for proteomics, although alternative chromatography methods are becoming more popular and may remain so for many years if improved over the years to address other l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
CPCG01N27/44773C07K1/26C07K1/285
Inventor M·科普R·桑多茨M·库尔西奥M·格劳瑟
Owner F HOFFMANN LA ROCHE & CO AG
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