37 results about "2 Dimensional Gel Electrophoresis" patented technology

Removal of abundant proteins from a sample enhances detection and resolution of less abundant proteins in the sample such as in two-dimensional gel electrophoresis. The removal is accomplished by immunosubtraction of several high abundance, interfering or contaminating proteins simultaneously.

The present invention provides methods for determining the methylation status of CpG-containing dinucleotides on a genome-wide scale using infrequent cleaving, methylation sensitive restriction endonucleases and two-dimensional gel electrophoretic display of the resulting DNA fragments. Such methods can be used to diagnose cancer, classify tumors and provide prognoses for cancer patients. The present invention also provides isolated polynucleotides and oligonucleotides comprising CpG dinucleotides that are differentially methylated in malignant cells as compared to normal, non-malignant cells. Such polynucleotides and oligonucleotides are useful for diagnosis of cancer. The present invention also provides methods for identifying new DNA clones within a library that contain specific CpG dinucleotides that are differentially methylated in cancer cells as compared to normal cells.

The present invention relates to protein markers for diagnosing stomach cancer and a diagnostic kit using the same, more precisely protein markers screened by two-dimensional gel electrophoresis and bioinformatics and a diagnostic kit using the same. The markers of the invention can be effectively used for diagnosing stomach cancer and evaluating the extent of progress of the cancer by confirming the expression levels of those marker proteins whose expressions differ in stomach cancer patients from in normal healthy people.

An electrophoresis assembly or disposable device for the separation of a complex protein sample, using two dimensional gel electrophoresis comprises a carrier such as a film or foil or glass plate (5) with an area for the first dimension gel strip (7) and an area (16) for the second dimension gel, where said two areas are directly in contact with each other. The assembly or device further comprises a two component main body made of a hard component (3) and a thin and flexible soft component layer (11) at a variable distance from the carrier (5) which body further comprises at least one slit or opening for external actuation of at least one valve (9, 10) where said valve is represented by said soft component material in correspondence of said at least one slit.

The present invention relates to a composition containing sHSPs for prevention of protein degradation and a composition for two-dimensional (2-D) gel electrophoresis. Furthermore, the present invention relates to the improved method of 2-D gel electrophoresis, which is characterized by using sHSPs. According to the present invention, decreasing of protein spots was prevented in the 2-D gel electrophoresis, thereby obtaining 2-D gel with much more protein spots.

The invention provides a goat milk proteome comparing method. The method includes the steps of a, collecting milk samples; b, preparing a solution; c, preparing skim milk protein samples; d, measuring the concentration of the sample protein, to be more specific, using a BCA kit, allowing PBS to be blank, using 5.0mg / mL BSA as the standard protein, and measuring the OD value of the sample protein with a microplate reader under the wavelength of 560 nanometers to obtain the protein concentration of the colostrum skim milk protein sample and the normal skim milk protein sample; e, performing two-dimensional gel electrophoresis, to be more specific, sequentially performing isoelectric focusing electrophoresis, gel strip equilibrium, SDS-PAGE vertical gel electrophoresis and gel image collecting and analysis to obtain the 2-DE spectrum of the colostrum milk sample and the normal milk sample. The goat milk proteome comparing method has the advantages that the protein components of biological systems can be analyzed in a large-scale manner at the same time, and a theoretical basis is provided for the systematic disclosing of the change law of goat milk proteome along with lactation stages.

The invention provides a kidney yin deficiency syndrome animal model biomarker screening and determining method. The method comprises the following steps: extracting a cortical bone total protein sample, measuring the protein concentration, carrying out bidirectional gel electrophoresis analysis, detecting the gel protein spot, displaying and analyzing differential protein spots, carrying out MALDI-TOF-MS of differential protein spots, searching the protein database to identify and determine differential proteins, identifying part of differential proteins through Western-Bolt and RT-PCR, and interactively verifying the identification results with ELISA detection results of differential proteins in the serum. The differential proteins, whose expression trends are consistent and are not in the normal value range, can be used as the biomarker for identifying a kidney yin deficiency syndrome animal model. The biomarker can be used as the discrimination index of kidney yin deficiency syndrome in animal experiments; and a base is provided for finding another kidney yin deficiency syndrome biomarker.