Goat Milk Proteome Alignment Method

Abstract

The invention provides a goat milk proteome comparing method. The method includes the steps of a, collecting milk samples; b, preparing a solution; c, preparing skim milk protein samples; d, measuring the concentration of the sample protein, to be more specific, using a BCA kit, allowing PBS to be blank, using 5.0mg / mL BSA as the standard protein, and measuring the OD value of the sample protein with a microplate reader under the wavelength of 560 nanometers to obtain the protein concentration of the colostrum skim milk protein sample and the normal skim milk protein sample; e, performing two-dimensional gel electrophoresis, to be more specific, sequentially performing isoelectric focusing electrophoresis, gel strip equilibrium, SDS-PAGE vertical gel electrophoresis and gel image collecting and analysis to obtain the 2-DE spectrum of the colostrum milk sample and the normal milk sample. The goat milk proteome comparing method has the advantages that the protein components of biological systems can be analyzed in a large-scale manner at the same time, and a theoretical basis is provided for the systematic disclosing of the change law of goat milk proteome along with lactation stages.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for comparing goat milk proteomes. Background technique [0002] Goat's milk has higher nutritional value than cow's milk, and its composition is more similar to that of breast milk, so it can be used as a good source of milk for infants and young children who are intolerant to cow's milk. [0003] Colostrum is the milk secreted by the female animal within 1 week, especially within 3 days after giving birth. The content of protein, fat and vitamins in colostrum is significantly higher than that in normal milk, especially colostrum contains more proteins with special functions than normal milk, such as immunoglobulins, blood complement system proteins, acute phase proteins, Proteins and peptides with direct antibacterial effects and biologically active proteins, etc. They play an important role in promoting the development of the gastrointestinal tract of young animals and resist...

AI Technical Summary

Problems solved by technology

However, these studies all use traditional biochemical methods to analyze the protein abundance changes of colostrum and normal milk, the analysis methods are limited, and there is a lack of systematic research on milk protein as a whole

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