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Method for making chronic multiple stress animal model

A technology of animal models and production methods, which can be applied to teaching models, educational tools, instruments, etc., and can solve problems such as inability to simulate stress effects

Inactive Publication Date: 2007-08-29
华中科技大学同济医学院
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Problems solved by technology

The production of chronic stress animal models reported in the existing literature usually adopts a single stressor, that is, binding, electric shock, pain, forced swimming, sleep deprivation or noise, etc., and the stress cycle is usually 3 weeks. This animal model It is impossible to simulate the stress effect produced by the living conditions of human beings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Using 37 male adult Wistar rats (provided by the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology), the Morris water maze was used to test the learning and memory performance of each animal for the first time (dl), and the animals with poor learning and memory performance were removed. Then they were randomly divided into normal control group (n=14; referred to as control group) and chronic compound stress group (n=23; referred to as stress group). The two groups of animals also received regular food and water. The temperature of the animal breeding room is 18-22°C, and the light-dark cycle is 12 / 12h.

[0047] Chronic compound stress consisted of 4 stressors including vertical rotation, sleep deprivation, binding and night light. Stressed animals were carried out for 6 weeks: vertical rotation, sleep deprivation and binding were performed during the day, 6h / d, one of which was performed every day. Programs are exchange...

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Abstract

This invention provides a preparation method of chronic composite stress animal model, and the specific operation is to make the adult Wistar rats to stress in four sources of vertical rotation, sleep deprivation, bundling and nighttime illumination for 6 weeks alternately without regulation, 6 hours per day. The animal model simulates the stress effect in human survival state, and can be used to study Neurobiological mechanism of rats' enhanced learning and memory capacity caused by complex chronic stress.

Description

technical field [0001] The invention relates to a method for making an experimental animal model, in particular to a method for making an animal model for medical experiments. Background technique [0002] With the development of society and economy, the competition is more intense, the pace of work and life is accelerated, and the interpersonal relationship is more complicated. The stress effect of these factors on people, especially the impact on cognitive function, has become the current neuroscience research field. one of the hot topics. Regarding the impact of chronic stress on learning and memory functions, many experimental results support the view that chronic stress damages learning and memory functions [see: 1. Lupien SJ, de Leon M, de Santi S, Convit A, Tarshish C, Nair NP, Thakur M, McEwen BS, Hauger RL, Meaney MJ. Cortisol levels during human aging predict hippocampal atrophy and memory deficits[J]. Nature Neurosci, 1998; 1:69-73.; 2, Lupien SJ, McEwen BS. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G09B23/36
Inventor 刘能保张敏海李晓恒孙臣友周艳玲
Owner 华中科技大学同济医学院
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