Antibody library screening method

Abstract

The present invention provides an improved method of screening a library of molecules to identify or select one or more members thereof which are candidate binding partners for one or more target entities: (a) contacting an expression library with one or more target entities; (b) subjecting said target entities to at least one washing step; (c) separating target entities which have become bound to one or more members of the expression library from unbound members of the expression library by separation through an organic phase, thereby separating candidate binding partners for said target entities from other library members.

Description

technical field

[0001] The present invention relates to methods of screening molecular libraries, especially phage display libraries, to identify or select one or more members thereof that are candidate binding partners (partners) for one or more targets, especially cell surfaces A candidate binding partner for a molecule. The invention further relates to improved methods of isolating candidate binding partners of said target from other library members. Background technique

[0002] Phage antibody display technology has been proved to be a very suitable technology for antibody screening and selection to identify target antigens (reviewed in Hoogenboom et al., 1998, Immunotechnol., 4: 1-20). A number of protocols rely on the availability of purified and / or recombinant antigens which are screened after being immobilized on solid supports.

[0003] Probing cell surface differentiation and identifying binding partners of cell surface molecules unique to certain cell types or d...

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