Method for tissue culture of Japanese premna

Abstract

A method for tissue culture of Japanese premna belongs to modern agricultural technology. The present invention comprises (1) preparation of explant: taking perennial Japanese premna branch to cut into stem nodes with scissors, and to guarantee each stem node having leaf bud; (2) asepsis operation: washing the stem nodes with water, dipping into alcohol, then taking out and dipping into solution of sodium hypochlorite, washing them with axenic water; (3) bourgeoning cultivation: stem nodes are inoculated into germination culture medium MSB + 6 -BA to germinating; (4) rooting culture: adopting rooting medium to process rooting culture; (5) after root system growing up, transplanting into organic soils, covering plastics cloth to train seedlings culturation and achieving living plant regeneration. The present invention can generate radicle of plant rapidly, increase the number of regenerating plants, shorten the time of new plant culturing. The method of this invention can achieve a great deal of Japanese premna plants rapidly, suits for varieties of Japanese premna industrial production and reproduction, and suit for extraction and application of effective components.

Description

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AI Technical Summary

Benefits of technology

This technology allows creating rootlets from olives that are fast growing but less expensive compared to other methods like cuttings or tissue culture techniques. By doing this it makes possible faster growth rates and more consistently produced crops without being affected by factors such as soil conditions during their initial stages of development. Overall, these technical results make smelling green vegetables (called olive oil) easier to grow on land and have potential benefits over existing sources due to its unique flavors.

Problems solved by technology

This patented describes how vibrissile material can be used for creation of new vegetables or other products from VLSCARA plants grown indoors over long periods of time without losing their quality due to poor conditions during winter months when they are still greenishly growing at night. However, there have been previous methods such as those described earlier (Baeker et al., 2004), which require multiple steps before starting up production. Additionally, current techniques involve culturing cells under specific environmental stress patterns until after harvesting them, but this process takes too much space and slow down productivity compared to more efficient ways like sown crops.