Anti-helicobacter pylori thallus, cell toxin egg yolk antibody product, preparation and application thereof

A technology against Helicobacter pylori and Helicobacter pylori, applied in the direction of antibodies, immunoglobulins from eggs, digestive system, etc., to achieve the effect of easy industrialization

Inactive Publication Date: 2007-10-24
林刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, there is no report on the use of Helicobacter pylori cells and cytotoxins to form multivalent antige...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Chicken yolk antibody products with antibody activity against Helicobacter pylori cells and cytotoxins can be obtained through the following steps: prepare multivalent antigens composed of Helicobacter pylori cells and cytotoxins, immunize healthy hens with this multivalent antigen, and then collect From the eggs it lays, anti-Helicobacter pylori cells and cytotoxic chicken egg yolk antibodies are extracted from the eggs.

Embodiment 2

[0022] Preparation of strains: Isolate Helicobacter pylori strains from the lesions of Helicobacter pylori-infected patients, and use the strain identification system to identify strains to obtain cytotoxin-producing strains. The cytotoxins produced include but not only include vacuolating toxin (VacA) and cytotoxin-like protein (CagA).

Embodiment 3

[0024] Preparation of Helicobacter pylori bacteria and cytotoxin multivalent antigen: inoculate the above bacteria into Brucella broth medium and culture for 72 hours, collect the bacteria and culture supernatant by centrifugation, and concentrate the culture supernatant by ultrafiltration to obtain cytotoxin . The bacterium is sterilized, the cytotoxin is detoxified, mixed in proportion, an equal volume of Freund's adjuvant is added, emulsified and mixed to obtain the multivalent antigen of Helicobacter pylori bacterium and cytotoxin.

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Abstract

The invention discloses an anti-pylorus screw thallus and chicken cytotoxin yolk antibody and application, which is characterized by the following: adopting pylorus screw thallus and cytotoxin form polyvalent antigen immune health hen; extracting anti-pylorus screw thallus and ytotoxin yolk antibody from yolk; fitting for treating relative disease infected by pylorus screw thallus.

Description

1. Technical field [0001] The invention relates to a product with antibody activity—anti-Helicobacter pylori bacterium and cytotoxic chicken egg yolk antibody, and relates to its manufacturing process and application. 2. Technical background [0002] In 1982, Australian scholars Barry Marshall and Robin Warren discovered Helicobacter pylori (Hp), which proved that the bacteria infecting the stomach would cause gastritis, gastric ulcer and duodenal ulcer, and thus obtained the 2005 Year Nobel Prize in Physiology and Medicine. [0003] Epidemiological survey data show that the infection rate of Hp in the Chinese population is about 40% to 60%. Due to the special living environment of Hp and the difficult access of drugs, it is very difficult to cure it. At present, triple therapy and quadruple therapy are mostly used in the clinical treatment of Hp infection, that is, antibiotics, antiviral drugs and acid-suppressing drugs are used in combination. However, the dosage of the...

Claims

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Application Information

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IPC IPC(8): C07K16/02A61K39/395A61P1/04
Inventor 林刚
Owner 林刚
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