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Method for separating and extracting Chinese Thorowax Root

A technology of Bupleurum and medicinal materials, applied in the field of medicine, can solve the problems of different extraction methods, discomfort of traditional Chinese medicinal materials, etc.

Inactive Publication Date: 2012-07-04
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction method of traditional Chinese medicine is the focus of research in the field of traditional Chinese medicine. Researchers have always focused their research on which method can be used to obtain more active ingredients from Chinese medicinal materials. Therefore, the current situation of extraction of Chinese medicinal materials is: for different The extraction methods of the effective components of Chinese herbal medicines are also different, and different extraction methods for different Chinese herbal medicines require a large number of different extraction equipment to support the extraction of Chinese herbal medicines, which is not suitable for the standardization of Chinese herbal medicine extraction

Method used

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  • Method for separating and extracting Chinese Thorowax Root
  • Method for separating and extracting Chinese Thorowax Root
  • Method for separating and extracting Chinese Thorowax Root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] (1) Extraction process: Weigh 250g of Bupleurum medicinal material, crush it and sieve it, then add 5 times of ethyl acetate and ethanol with a volume ratio of 1:1, heat and reflux for 2 hours, extract 3 times, and combine the filtrates to obtain Extract fr.1. Add 10 times the amount of 70% ethanol to the dregs, heat and reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract fr.2. Finally add water to the dregs, heat and reflux for 1.5 hours, extract twice, and combine the filtrates to obtain the extract fr.3.

[0097] (2) Separation process: Concentrate the extract fr.1 to obtain 35.6g extract, mix the sample with silica gel, and use a normal phase silica gel column to separate it. First, petroleum ether and ethyl acetate with a volume ratio of 50:1 are used as Mobile phase, to obtain eluent fr.4, then change the volume ratio of chloroform and methanol as mobile phase to obtain eluent fr.5, concentrate to obtain 5.8g sample, finally use meth...

Embodiment 2

[0128](1) Extraction process: Weigh 500g of Radix Bupleurum medicinal material, crush it and sieve it, then add 10 times of ethyl acetate and ethanol with a volume ratio of 1:1, heat and reflux for 3 hours, extract twice, and combine the filtrates to obtain Extract fr.1. Add 8 times the amount of 70% ethanol to the dregs, heat and reflux for 2 hours, extract 3 times, and combine the filtrates to obtain the extract fr.2. Finally add water to the dregs, heat and reflux for 2.5 hours, extract twice, and combine the filtrates to obtain the extract fr.3.

[0129] (2) Separation process: Concentrate the extract fr.1 to obtain 70.6g extract, mix the sample with silica gel, and use a normal phase silica gel column to separate it. First, petroleum ether and ethyl acetate with a volume ratio of 50:1 are used as Mobile phase, to obtain eluent fr.4, then change the volume ratio of chloroform and methanol as mobile phase to obtain eluent fr.5, concentrate to obtain 5.3g sample, finally us...

Embodiment 3

[0160] 1. Extraction and separation of Bupleurum medicinal materials

[0161] (1) Extraction process: Weigh 250g of Radix Bupleurum medicinal material, crush it and sieve it, then add 8 times of ethyl acetate and ethanol with a volume ratio of 1:1, heat and reflux for 1 hour, extract twice, and combine the filtrates to obtain Extract fr.1. Add 8 times the amount of 70% ethanol to the dregs, heat and reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract fr.2. Finally add water to the dregs, heat and reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract fr.3.

[0162] (2) Separation process: Concentrate the extract fr.1 to obtain 36.6g of extract, mix the sample with silica gel, and use a normal phase silica gel column to separate it. First, petroleum ether and ethyl acetate with a volume ratio of 50:1 are used as Mobile phase, to obtain eluent fr.4, then change the volume ratio of chloroform and methanol as mobile phase ...

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Abstract

The invention is concerned with the standard extraction and isolation method of Bupleurum. It can form a medicinal materials component store according to different polarity levels of the Bupleurum divide into several groups, which each group contents few types of compound. It can use to discover new medicine by conducting drug screening from the component store. The method can reduce the period of medicine extraction and isolation, and reduce the consumption of manpower and material resources, and it suits for most types of medicine.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the extraction and separation standardization of Radix Bupleurum. Background technique [0002] At present, Chinese herbal medicine has a certain market all over the world. With the increase of people's awareness of health requirements and the aging of the population, sub-health status, people are more eager to return to nature, and use pure natural medicine with high degree of treatment, To prevent problems that cannot be solved by some chemically synthesized drugs, the application of natural herbal medicines is beyond the background of its original national traditional culture. It has become the goal pursued by pharmaceutical companies all over the world to seek medicines with low side effects and high quality and low price from natural medicines. The European Community has carried out unified legislation on herbal medicines, and the status of herbal medicines in countries ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/233C07B63/00
Inventor 程翼宇贺庆王毅王学伟李云飞水文波胡兴江葛志伟
Owner TIANJIN TASLY PHARMA CO LTD