Monoamine oxidase diagnosing reagent kit and method for measuring monoamine oxidase activity concentration

Abstract

The invention relates to a monoamine oxidase diagnosis reagent box of Enzymatic Recycling Method, and the invention also relates to method principle for detecting the solution of monoamine oxidase, constitute and component of reagent, which belongs to the technical field of medical checking measurements. The reagent box in the invention can be dry powder state, and used after dissolution; it also can be formulated to be liquid agent for direct usage. The component of reagent box mainly contains: buffering liquid, alpha- ketoglutaric acid, glutamate dehydrogenase EC 1.4.1.2, EC 1.4.1.3, EC 1.4.1.4, Glutamate oxidase EC 1.4.3.7, EC 1.4.3.11, reduction type coenzyme and stabilizer, and the component can be matched to form single-reagent reagent box, two-reagent reagent box, and three- reagent box; The enzymatic reaction is generated between sample and reagent by mixing sample and reagent with definite volume, then the reactants is placed under the ultraviolet / visible light analyzer to detect ascend degree of absorbance at main wavelength of 340nm, so concentration of monoamine oxidase can be calculated. The invention can obtain the detecting result by ultraviolet / visible light analyzer, and the sensibility is high, precision is well, and easy to be spread.

Description

technical field

[0001] The invention relates to a diagnostic kit for monoamine oxidase, and at the same time, the invention also relates to a method for measuring the activity concentration of monoamine oxidase, which belongs to the technical field of medical examination and measurement. Background technique

[0002] The determination of monoamine oxidase (MAO) can be divided into chemical spectrophotometry, fluorescence method, immunosuppressive method and enzymatic method which is now applying for a patent. Generally, chemical spectrophotometry is used. The substrates of monoamine oxidase are benzylamine and p-benzylamine-β-azonaphthol. Monoamine oxidase can also be used to catalyze the release of H from amines. 2 o 2 Oxidative chromogens such as 10-N-methylcarbamoyl-3,7-dimethylamino-10-hydro-phenothiazine (MCDP). In addition, the reaction substrates of monoamine oxidase include butylamine, amylamine, b-phenylethylamine, tyramine (3-p-hydroxyphenylethylamine 3 -parahdr...