Method for preparing feed additive based on natural plant active component biotransformation
A technology of feed additives and natural plants, which is applied in the field of feed additives to achieve the effects of simple drug efficacy, improved feed conversion rate, and better formulation compatibility.
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Embodiment 1
[0052] Example 1: The crude extracts of dandelion and astragalus were biotransformed to produce feed additives by different microorganisms.
[0053] Take Aspergillus niger and Bacillus subtilis and store them at 4°C;
[0054] Step 1: Biotransformation of crude extract of dandelion by Aspergillus niger
[0055] This example is mainly to increase the content of total flavonoids in active ingredients of dandelion, so as to improve the immunity of livestock and poultry, and promote the growth of animals.
[0056] (1) Preparation of Aspergillus niger seeds and fermentation medium
[0057] Inoculate Aspergillus niger in the seed expansion medium, put it in a constant temperature incubator at 35°C-40°C and cultivate it for 2-3 days, then insert the seed optimized fermentation medium and place it in a constant temperature shaking table at 35°C-40°C Cultivate in the box for 2-3 days, and fill each 300ml triangular bottle with 50ml liquid medium to obtain the cultured Bacillus subtili...
Embodiment 2
[0084] Example 2: Production of feed additives by solid fermentation of dandelion and astragalus by different microorganisms
[0085] Step 1: Fermentation of dandelion solid by Aspergillus niger
[0086] This example is mainly to increase the content of total flavonoids in active ingredients of dandelion, so as to improve the immunity of livestock and poultry, and promote the growth of animals.
[0087] (1) the preparation of Aspergillus niger seed and seed optimization fermentation medium is identical with embodiment 1;
[0088] (2) Preparation of Dandelion
[0089] Pulverize the dandelion to 80 orders with an ultrafine pulverizer, then soak it with 35% water, and set aside.
[0090] (3) Fermentation of dandelion solids by Aspergillus niger
[0091] Mix 20% of Aspergillus niger agent with 2:1:1 ratio of aminoacetic acid, urea, 20% of inorganic salts and 60% of dandelion evenly, put it into a ventilated tank, ferment for 5 days at 35-40°C, extract with water, alcohol Sink,...
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