Preparation method of dextran monoclonal antibody

A monoclonal antibody and dextran technology, applied in biochemical equipment and methods, anti-animal/human immunoglobulin, microorganisms, etc., can solve the problem of low immunogenicity of dextran, achieve novel technology, reduce The cost of sugar production and the effect of rich sources of raw materials

Inactive Publication Date: 2008-03-12
GUANGZHOU SUGARCANE IND RES INST
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Problems solved by technology

[0005] The purpose of the present invention is to propose a preparation method of dextran monoclonal antibody for rapid determination of dextran content in sugar products using the principle of immunology, which can improve the immunogenicity of dextran and solve the problem of dextran immunity. Key technical issues of low originality

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  • Preparation method of dextran monoclonal antibody

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Embodiment Construction

[0024] The present invention is illustrated below through specific examples.

[0025] A preparation method of glucan monoclonal antibody, it comprises the steps:

[0026] A. Selection and its coupling

[0027] Using new carrier coupling technology and immunization scheme, keyhole limpet hemoglobin and bovine serum albumin were selected to be coupled with dextran antigen respectively to obtain keyhole limpet hemoglobin-dextran antigen (hereinafter referred to as antigen 1) and bovine serum albumin-glucan antigen. Glycan antigen (hereinafter referred to as antigen 2).

[0028] B. Immunization and blood test

[0029] Select 5 female BALB / c mice aged 4-8 weeks, take Antigen 1 and complete Freund's adjuvant (CFA) to fully mix and emulsify to form a "water-in-oil" structure, and inject them subcutaneously at multiple points on the back of the mice respectively. The dose of antigen injected into each mouse was 40-50 μg. The second immunization was performed after an interval of 3...

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Abstract

The present invention relates to the immunology and sugar technology, aiming to take advantage of the immunology principles to measure the glucan content in the sugar and to propose a preparation method for glucan monoclonal antibody. The present invention comprises the following steps: A. The selection and coupling of antigen. Shikongqi hemoglobin and bovine serum albumin are respectively coupled with glucan to generate antigen; B. Animal immunity and blood test; C. cell fusion and cultivation; D. Hybridoma screening; E. Hybridoma cloning; F. preparation of hybridoma ascites. The present invention is of substantive features and notable progress: 1. rich raw materials, 2. safe and reliability 3. Glucan-specific monoclonal antibody can be gained and can be substantially cultivated; glucan-specific monoclonal antibody can be easily used to achieve sandwich ELISA immunology kit that can quantitatively measure the glucan. The present invention makes the fast and accurate measurement of glucan in sugar cane source, manufactured products possible.

Description

technical field [0001] The present invention relates to immunology and sugar production technology. Background technique [0002] Dextran is a linear polymer of glucose molecules with the molecular formula (C 6 h 10 o 5 ) n , the molecular weight is indeterminate, generally between tens of thousands to several million, it is sticky, and the viscosity increases with the increase of molecular weight and concentration. Dextran is generally soluble in water, sucrose solution, sodium chloride solution and ethanol solution, and the solubility decreases with the increase of molecular weight and concentration. In sugar cane and cane juice, glucans are formed by the polymerization of glucose by enzymes produced by microorganisms. Dextran troubles are present throughout the sugar process, from cane juice to refined products. Dextran increases the optical rotation of raw sugar, resulting in "false optical rotation"; dextran is extremely harmful to the refining of raw sugar, it si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C12N5/18
Inventor 梁达奉戚荣陈海宁郭剑雄余构彬
Owner GUANGZHOU SUGARCANE IND RES INST
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