Transgene enterobacter cloacae CEI-a and its construction method and use

A kind of Enterobacter cloacae, transgenic technology, applied in biological field
CN101144067BInactive Publication Date: 2011-08-10GUANGDONG INST OF APPLIED BIOLOGICAL RESOURCES
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGDONG INST OF APPLIED BIOLOGICAL RESOURCES
Publication Date
2011-08-10
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The present invention provides transgenic enterobacter cloacae (Enterobacter cloacae) GEI-a, and the construction method and the purpose thereof. The bacterial strain utilizes a Tn5 transposon to insert a soybean serine proteinase inhibitor gene into the genome DNA of the enterobacter cloacae, to be used to express engineering strain which inhibits the insect intestinal tract trypsin. The strain preserves in the China center for type culture collection, and the serial number is CCTCC M 207071. Fire ant symbiotic bacteria for expressing an exogenous gene is used as a bait to feed the fire ants, recombinant bacterium reproduces in the intestine cavity of the fire ant, and the albumen which can cause the death of the fire ant is produced. The present invention is the secure and effective method to incessantly control the fire ants.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a transgenic Enterobacter cloacae strain, its construction method and its application. Background technique

[0002] Plant protease inhibitors (Protein inhibitor, PI for short) are a class of low-molecular-weight polypeptides or proteins that widely exist in the plant kingdom, and have inhibitory effects on insect proteases. When protease inhibitors are fed by insects and accumulate in the body, it can bind to the active site or allosteric site of insect protease, inhibit the catalytic activity of protease, disrupt the normal metabolism of insects, cause insect indigestion, and cause a lack of essential amino acids. Thereby, the growth and development of insects are hindered, and even die. Thus these inhibitors have great potential in pest control [Bowler1990 Defense-related proteins in higher plants. Annual Review of Biochemistry, 59: 873-907; Liu Wuge et al. 2000 Prote...

Claims

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