Application of lanthanum trioxide during growth of Tiansan saussurea involucrata seedling
A technology of lanthanum trioxide and snow lotus, applied in the fields of application, plant regeneration, plant cells, etc., can solve the problems of seedling death, slow growth, low reproductive efficiency, etc., shorten the growth cycle, promote efficient rooting, and improve growth quality Effect
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Embodiment 1
[0021] 1. Prepare 200 micromoles per liter of La 2 o 3 solution, add 0.5% agar powder, and sterilize at 121°C for 17 minutes. Get the uniform and strong Tianshan Snow Lotus rootless tissue culture seedlings (long 2cm) in the growth state and transfer them to plants containing La 2 o 3 On the agar medium, each culture bottle was transferred with 3 to 5 plants, and then placed in a culture room with a temperature of 25±2°C, a light intensity of 4000 Lux, and a photoperiod of 16 hours.
[0022] 2. After 35 days of cultivation, the rooting frequency reaches 100%, with an average of 6 roots on each seedling and an average root length of 2.5cm, which is better than that without adding La 2 o 3 solution medium. Take the rooted seedlings out of the culture bottle, wash off the attached agar with tap water, and then transfer to the perlite support culture medium, and use a concentration of 200 micromole per liter of La 2 o 3 Water the roots with the solution, cover them with sha...
Embodiment 2
[0025] 1. Take high-quality peat and place it in a culture bottle (height is 2 / 5 of the culture bottle), and then add a concentration of 200 micromoles per liter of La 2 o 3 The solution was sterilized at 121°C for 30 minutes until no water accumulated. Get the uniform and strong Tianshan Snow Lotus rootless tissue culture seedlings (long 2cm) in the growth state and transfer them to plants containing La 2 o 3 On the agar medium, each culture bottle was transferred with 3 to 5 plants, and then placed in a culture room with a temperature of 25±2°C, a light intensity of 4000 Lux, and a photoperiod of 16 hours.
[0026] 2. After 35 days of cultivation, the rooting frequency reached 95%, with an average of 8 roots per seedling and an average root length of 2.9cm, which is better than that without adding La 2 o 3 solution medium. The rooted seedlings were taken out of the culture bottle, and then transferred to the perlite support culture medium, with a concentration of 200 mi...
Embodiment 3
[0029] 1. Take perlite: peat: vermiculite volume ratio of 3:1:1 mixed matrix, sterilized with 800 times of carbendazim, the next day, take Tianshan Snow Lotus rootless tissue culture seedlings (long 2cm) transfer to In the mixed matrix, La 2 o 3 Water the roots with the solution, cover them with shade nets, and spray tap water once every hour, preferably with air humidity not lower than 80%. The temperature is 25±2°C, the light intensity is 2500Lux, and the photoperiod is 16 hours.
[0030] 2. After cultivating for 7 days, remove the shading net, and use La at a concentration of 200 micromoles per liter 2 o 3 The solution is sprayed on the leaves until droplets are formed but no water drips down. Adjust the interval of each spraying of tap water, preferably with air humidity not lower than 50%. After that, spray La at a concentration of 100 micromole per liter at intervals of one week 2 o 3 solution. After one month, the survival rate of the seedlings was 72%, and the r...
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