Milk containing placenta polypeptide extraction and preparation method thereof
A technology of placental polypeptides and extracts, applied in food preparation, milk preparations, dairy products, etc., can solve problems such as no research reports, and achieve the goal of alleviating restlessness, good economic and social benefits, and enhancing human physique Effect
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Embodiment 1
[0040] Collect bovine placenta tissue; wash the fresh bovine placenta tissue with purified water at room temperature; add 4 times the volume of sterile ultrapure water to make a homogenate; add 5 times the volume of sterile ultrapure water, mix well, and set- Repeated freezing and thawing twice at 20°C; centrifuge the frozen-thawed homogenate at a temperature of 4-8°C at a speed of 8000 rpm for 20 minutes to remove the precipitate; intercept the supernatant with a tangential flow ultrafiltration device, and take the molecular weight < 30,000 daltons of placental polypeptide extract, wherein the content of polypeptides with a molecular weight lower than 80 daltons in the placental polypeptide extract is 57.0% to 69.3%, the content of polypeptides with a molecular weight of 90 to 280 daltons is 20.1% to 31.7%, 280 ~30,000 Daltons contain 5.2-11.3% peptides, sterilize and filter, and reserve; add 1ml of placental polypeptide extract to 200ml of fresh milk, process through high-pre...
Embodiment 2
[0042] Collect bovine placenta tissue; wash the frozen fetal bovine placenta tissue with ultrapure water at room temperature; add 5 times the volume of sterile ultrapure water to make a homogenate; add 4 times the volume of sterile ultrapure water and mix well , placed at -15°C and repeated freezing and thawing 3 times; centrifuge the frozen-thawed homogenate at 4-8°C at 8,000 rpm for 15 minutes to remove the precipitate; intercept the supernatant with a tangential flow ultrafiltration device, and take Placental polypeptide extract with a molecular weight of less than 30,000 Daltons, wherein the content of polypeptides with a molecular weight lower than 80 Daltons in the placental polypeptide extract is 57.0% to 69.3%, and the content of polypeptides with a molecular weight of 90 to 280 Daltons is 20.1% to 31.7%. , 280-30,000 Daltons, the peptide content is 5.2-11.3%, sterilized and filtered, and set aside; add 1ml of placental polypeptide extract to 200ml of fresh milk, proces...
Embodiment 3
[0044]Collect bovine placenta tissue; wash the fresh bovine placenta tissue with ultrapure water at room temperature; add 3 times the volume of sterile ultrapure water to make a homogenate; add 7 times the volume of sterile ultrapure water, mix well, Place at -25°C and freeze-thaw twice; centrifuge the frozen-thawed homogenate at 4-8°C at 8,000 rpm for 25 minutes to remove the precipitate; intercept the supernatant with a tangential flow ultrafiltration device, and take the molecular weight <30000 Dalton placental polypeptide extract, wherein the content of polypeptides with a molecular weight lower than 80 Daltons in the placental polypeptide extract is 57.0% to 69.3%, and the content of polypeptides with a molecular weight of 90 to 280 Daltons is 20.1% to 31.7%, The peptide content of 280-30000 Daltons is 5.2-11.3%, sterilized and filtered, and set aside; add 1ml of placental polypeptide extract to 200ml of fresh milk, process through high-pressure homogenization process, and...
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