Method for degrading kelp polysaccharide sulfate

The technology of kelp sulfate polysaccharide and kelp is applied in the field of marine biology, can solve the problems of acid polysaccharide sulfate group shedding, high requirements on equipment and equipment, and cannot be applied to industrial production, etc., and achieves fast degradation speed, less reagent consumption and low cost. Effect

Inactive Publication Date: 2010-05-26
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] 2. Alkaline hydrolysis: Under alkaline conditions, it often causes the modification of acidic polysaccharides and the loss of sulfate groups, which affects the activity of the product, so it is not suitable for seaweed sulfated polysaccharides
These two methods are currently unable to be applied to industrial production due to high energy consumption, high requirements for equipment conditions, and small sample processing volume.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Separation and purification of laminar sulfate polysaccharide

[0027] Take kelp and soak it in water for 2 hours, and the swollen kelp is crushed into kelp pulp. Extract by heating at 95°C for 6h, cool to room temperature and filter. Add calcium chloride to the filtrate, and centrifuge to remove calcium alginate. A 3% (mass fraction) CTAB (cetyltrimethylammonium bromide) solution was added to the supernatant to combine with LPS for precipitation, and the added mass of CTAB was 40% of the polysaccharide mass. Centrifuge at 3000r / min for 10min to collect the CTAB-LPS precipitate. Calcium chloride is added for salt solution to free LPS, then ethanol is added to precipitate LPS, and pure LPS is obtained by centrifugation and drying. Its average molecular weight is 172,000, mainly block copolymers composed of α-1,4-L guluronic acid and β-1,4-D-mannuronic acid as monomers, α-L-fucus Polymers of sugar-4-sulfate and polymers of β-D-glucopyranose, the content of sulfate...

Embodiment 2

[0031] (1) Separation and purification of laminar sulfate polysaccharide

[0032] Take kelp and soak it in water for 2 hours, and the swollen kelp is crushed into kelp pulp. Heat and extract at 100°C for 2h, cool to room temperature and filter. Add calcium chloride to the filtrate, and centrifuge to remove calcium alginate. A 4% (mass fraction) CTAB (cetyltrimethylammonium bromide) solution was added to the supernatant to combine with LPS for precipitation, and the added mass of CTAB was 110% of the polysaccharide mass. Centrifuge at 3000r / min for 10min to collect the CTAB-LPS precipitate. Calcium chloride is added for salt solution to free LPS, then ethanol is added to precipitate LPS, and pure LPS is obtained by centrifugation and drying. Its average molecular weight is 253,000, mainly block copolymers composed of α-1,4-L guluronic acid and β-1,4-D-mannuronic acid as monomers, α-L-fucus Polymers of sugar-4-sulfate and polymers of β-D-glucopyranose, the content of sulfate...

Embodiment 3

[0036] (1) Separation and purification of laminar sulfate polysaccharide

[0037] Take kelp and soak it in water for 2 hours, and the swollen kelp is crushed into kelp pulp. Extract by heating at 100°C for 4h, cool to room temperature and filter. Add calcium chloride to the filtrate, and centrifuge to remove calcium alginate. A 5% (mass fraction) CTAB (cetyltrimethylammonium bromide) solution was added to the supernatant to combine with LPS for precipitation, and the amount of CTAB added was 150% of the mass of the polysaccharide. Centrifuge at 3000r / min for 10min to collect the CTAB-LPS precipitate. Calcium chloride is added for salt solution to free LPS, then ethanol is added to precipitate LPS, and pure LPS is obtained by centrifugation and drying. Its average molecular weight is 1.5 million, mainly block copolymers composed of α-1,4-L guluronic acid and β-1,4-D-mannuronic acid as monomers, α-L-fucus Polymers of sugar-4-sulfate and polymers of β-D-glucopyranose, the con...

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PUM

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Abstract

The invention discloses a degradation method of kelp sulfuric acid polysaccharide. The invention is characterized in that the kelp sulfuric acid polysaccharide is abstracted from kelp, and then is degraded thorough hydrogen peroxide to obtain low molecular weight kelp sulfuric acid polysaccharide. The degradation speed of the method is quick, the reagent use level is extremely low, the process issimple, the cost is inexpensive, the extra hydrogen peroxide is easy to be analyzed and removed, the content of sulfate group and total sugar basically keeps unchangeable, the distribution of the molecular weight is concentrated, and the range of the molecular weight can be controlled. Because of the bleaching action of the hydrogen peroxide, the appearance of the low molecular weight kelp sulfuric acid polysaccharide is fleckless.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, in particular relates to the research and development of marine medicines, in particular to a method for degrading kelp sulfated polysaccharides. Background technique [0002] Laminaria polysaccharide sulfate (LPS) is a water-soluble heteropolysaccharide containing a variety of monosaccharides and sulfate groups. The content of LPS in kelp varies with its type, origin, season and different parts of the plant. LPS has a variety of biological functions, such as anti-tumor, anti-virus, hypoglycemic and lipid-lowering effects, immune regulation and regulation of cell proliferation. [0003] The structure, monosaccharide composition and sulfate group content of LPS from different sources are different. The biological activity of LPS is not only related to the content of sulfate groups in the chain, but also closely related to its molecular weight. The larger the molecular weight and volume ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 欧阳健明王淼
Owner JINAN UNIVERSITY
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