Method for preparing coriolan

Abstract

The invention relates to a novel preparation method of coriolus versicolor polysaccharide (CVPS), which is characterized in that the mycelium of coriolus versicolor is immerged in hot water based on a mass ratio of 1:4, extraction and filtration are made repeatedly for twice, then water extract is combined and concentrated to one third to one fifth of the original volume; ethanol is added and kept static for sedimentation and then filtered; the sediment is dried to prepare crude amylase to be dissolved with hot water and then added with chloroform and n-butyl alcohol and surged fiercely till jello is generated; centrifugal separation is carried out to gather supernatant fluid; pH is adjusted to be equal to 9.0 by NaOH, then H2O2 is dripped into the water bath with the temperature of 50 DEG C and the pH is kept between 8.0 to 9.0; the water bath lasts for 4h and the reaction stops and ethanol is added slowly; after that, the supernatant fluid is put into a refrigerator for the night and sediment is gathered and dried on next day to get product. The invention can obviously promote the yield of the CVPS, remarkably shorten operation time, reduce the consumption of energy and ensure that the CVPS with relatively higher activity for batch production with less production cost is produced, thus resulting in the commercialization of the CVPS on a larger industrialization degree.

Description

technical field [0001] The invention relates to a new preparation method of versicolor versicolor polysaccharide. Background technique [0002] Coriolus versicolor is a precious resource of Chinese herbal medicine in my country. Coriolus versicolor polysaccharides (Coriolus versicolor polysaccharides, CVP) can be produced in vivo and in vitro, which is a protein-containing glycopeptide complex. β(1,4), β(1,3) or β(1,4), β(1,6)-based glucan complexes, with β(1,3) or β(1,6 ) combined hair branch, the polysaccharide content is 30% to 60%, and the binding protein is 15%. [0003] Yunzhi polysaccharide can activate immune cells, improve the immune function of the body, and significantly improve the intensity of the body's inflammatory response. It has various biological activities such as anti-tumor, anti-ulcer, lowering blood fat, and protecting the liver. Stimulate the transformation of macrophages, increase the effect of interleukin 1, interferon gamma and tumor necrosis facto...

AI Technical Summary

Problems solved by technology

[0004] At present, the methods of extracting polysaccharides from Yunzhi polysaccharides are mainly divided into water extraction method, acid extraction method, and alkali extraction method according to different extraction solvents. The difference is not big, but it will seriously pollute the environment, while the water extraction method has strong advantages in terms of production process, production cost, product safety and environmental protection.

[0005] The current water extraction method often uses the method of heating and boiling the fruiting bodies of Yunzhi to extract polysaccharides. This process alone takes 5 to 10 hours, or even longer time, and the amount of extracting liquid is relatively large, sometimes as high as 30 times. The process consumes a lot of energy, and the yield of polysaccharides from Yunzhi polysaccharide is only about 7.81%.

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