Method for preparing coriolan

A technology of Yunzhi polysaccharide and crude polysaccharide is applied in the field of preparation of Yunzhi polysaccharide, which can solve the problems of large energy consumption, large consumption, environmental pollution and the like, and achieve the effects of reducing energy consumption, reducing production cost and improving yield.

Active Publication Date: 2008-05-21
GUANGDONG INST OF MICROORGANISM +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the methods of extracting polysaccharides from Yunzhi polysaccharides are mainly divided into water extraction method, acid extraction method, and alkali extraction method according to different extraction solvents. The difference is not big, but it will seriously pollute the environment, while the water extraction method has strong advantages in terms of production process, production cost, product safety and env

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  • Method for preparing coriolan

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Experimental program
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Effect test

Embodiment 1

[0020] Weigh the net weight of Versicolor versicolor mycelium 122.13g (dried weight 16.22g), add 125mL of pure water and crush it, add water to 350mL, then add 7gNa 2 CO 3 , after 2 hours of water bath at 90°C, repeated leaching and filtration twice, and then the extract was concentrated to 1 / 5 of the original volume. 200 mL of the obtained concentrated solution was added with 3 times the volume of 95% ethanol for precipitation, left to stand for 24 hours, filtered, and the precipitated part was dried to obtain crude polysaccharide. Add 200mL of 70℃ hot water to dissolve the crude polysaccharide, add 40mL of chloroform and 8mL of n-butanol, shake vigorously for 20min to form a gel, and filter at 4000r / min in a centrifuge for 15min, collect the supernatant, remove the denatured protein in the lower layer and the chloroform in the upper layer , to obtain a solution of 130 mL. Adjust the pH to 9.0 with NaOH, add 15% H 2 o 2 20mL, and then in a water bath at 50°C for 4h, and s...

Embodiment 2

[0021] Embodiment 2: the ultraviolet absorption spectrometry of the product that embodiment 1 obtains

[0022] The ultraviolet scanner HITACHI (Japan) carried out ultraviolet scanning of 190-360nm on the sample aqueous solution to determine whether it is a protein-bound polysaccharide. The measurement results are shown in Figure 1. Yunzhi polysaccharide is a protein-containing glycopeptide complex. It can be seen from the figure above that there is no absorption at 260nm, and there is a shoulder-shaped peak at around 270-280nm, which is the characteristic absorption of protein-bound polysaccharides.

Embodiment 3

[0023] Embodiment 3: the Yunzhi polysaccharide content determination of the product that embodiment 1 obtains

[0024] Accurately weigh 20mg of standard glucose in a 500mL volumetric flask, add water to the mark, absorb 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6, 1.8mL respectively, make up to 2.0mL with water, and then add 5% Phenol 1.0mL and concentrated sulfuric acid 5.0mL, let stand for 10min, shake well, 30℃ water bath for 20min. After that, the optical density (OD) was measured at 490nm, and 2.0mL of water was used as a blank. Abscissa is glucose concentration, and ordinate is OD value, draws standard curve figure (seeing accompanying drawing 2), carries out linear regression, obtains regression equation: y=0.0146x+0.0103, R 2 = 0.9984. Draw 1.0ml of the sample solution, the operation steps are the same as above, repeat three times, the measured OD values ​​are 0.2983, 0.2901, 0.2956, take the average value, and check the standard curve. The correction coefficient of Yunz...

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Abstract

The invention relates to a novel preparation method of coriolus versicolor polysaccharide (CVPS), which is characterized in that the mycelium of coriolus versicolor is immerged in hot water based on a mass ratio of 1:4, extraction and filtration are made repeatedly for twice, then water extract is combined and concentrated to one third to one fifth of the original volume; ethanol is added and kept static for sedimentation and then filtered; the sediment is dried to prepare crude amylase to be dissolved with hot water and then added with chloroform and n-butyl alcohol and surged fiercely till jello is generated; centrifugal separation is carried out to gather supernatant fluid; pH is adjusted to be equal to 9.0 by NaOH, then H2O2 is dripped into the water bath with the temperature of 50 DEG C and the pH is kept between 8.0 to 9.0; the water bath lasts for 4h and the reaction stops and ethanol is added slowly; after that, the supernatant fluid is put into a refrigerator for the night and sediment is gathered and dried on next day to get product. The invention can obviously promote the yield of the CVPS, remarkably shorten operation time, reduce the consumption of energy and ensure that the CVPS with relatively higher activity for batch production with less production cost is produced, thus resulting in the commercialization of the CVPS on a larger industrialization degree.

Description

technical field [0001] The invention relates to a new preparation method of versicolor versicolor polysaccharide. Background technique [0002] Coriolus versicolor is a precious resource of Chinese herbal medicine in my country. Coriolus versicolor polysaccharides (Coriolus versicolor polysaccharides, CVP) can be produced in vivo and in vitro, which is a protein-containing glycopeptide complex. β(1,4), β(1,3) or β(1,4), β(1,6)-based glucan complexes, with β(1,3) or β(1,6 ) combined hair branch, the polysaccharide content is 30% to 60%, and the binding protein is 15%. [0003] Yunzhi polysaccharide can activate immune cells, improve the immune function of the body, and significantly improve the intensity of the body's inflammatory response. It has various biological activities such as anti-tumor, anti-ulcer, lowering blood fat, and protecting the liver. Stimulate the transformation of macrophages, increase the effect of interleukin 1, interferon gamma and tumor necrosis facto...

Claims

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Application Information

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IPC IPC(8): A23L1/28A23L1/29A23L1/09A23L33/00
Inventor 许国焕龚全吴月嫦付天玺江永明孟鹏
Owner GUANGDONG INST OF MICROORGANISM
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