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Method for preparing forsythiaside A

A forsythiaside and drying technology, applied in the field of preparation of forsythiaside A, can solve the problems of difficult regeneration and reuse of separation materials, expensive separation materials, low efficiency and the like, and achieves easy elution and regeneration, simple refining process, simple craftsmanship

Inactive Publication Date: 2010-11-03
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above extraction methods are inefficient, use a large amount of toxic organic solvents, and the separation materials used are not easy to regenerate and reuse, so they cannot be transplanted and scaled up for industrial production
In 2006, Chinese scholars successively applied for three Chinese patents (200610027024.1, 200610027036.4, 200610027037.9) of "purifying forsythiaside A from forsythia extract". This method is suitable for the preparation of small samples, and the separation materials used are expensive
In 2004, our research group obtained a patent (ZL 01122779.6) on the separation and purification of forsythiaside by using macroporous adsorption resin. Up to about 60%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 400g of dry forsythia shell, extract twice with 8Kg 50% ethanol solution, extraction temperature is 60°C, extraction time is 60min, combine the extracts, filter, the filtrate is concentrated under reduced pressure at 60°C to a water extract with a specific gravity of 1.08; water The extract was separated on a polyamide column (diameter-to-height ratio: 1:15), and the loading volume was 1:5. First rinse with deionized water until the effluent is brown-yellow, then rinse with 30% ethanol for 3 times the column volume, collect the 30% ethanol eluent, concentrate under reduced pressure to a specific gravity of 1.08 to obtain a preliminary separation concentrate; preliminary separation The concentrated solution was refined on an MDS column (diameter-to-height ratio: 1:15), first rinsed with deionized water until the eluate was pale yellow, then rinsed with 20% ethanol for 4 column volumes, and collected 20% ethanol. lotion. Thin-layer chromatography detection (developi...

Embodiment 2

[0023] Take 400g of dried forsythia shells, extract twice with 15Kg deionized water, extraction temperature is 80°C, extraction time is 45min, the extracts are combined, filtered, and the filtrate is concentrated under reduced pressure at 60°C to a water extract with a specific gravity of about 1.05; the water extract Separation on a D101 column (diameter-to-height ratio: 1:15), with a loading volume of 1:4. First rinse with deionized water until the effluent is brown-yellow, then use 20% ethanol to rinse for 4 times the column volume, collect the 20% ethanol eluate, concentrate under reduced pressure to a specific gravity of 1.05 to obtain a preliminary separation concentrate; The concentrated solution was refined on an MDS column (diameter-to-height ratio: 1:12), rinsed with deionized water until the eluate was pale yellow, then rinsed with 30% ethanol for 4 column volumes, and collected 30% ethanol. lotion. Thin-layer chromatography detection (developing solvent is methyl ...

Embodiment 3

[0025] Take 1Kg of dry forsythia shell, extract twice with 35Kg 40% ethanol solution, extraction temperature is 80°C, extraction time is 80min, combine the extracts, filter, the filtrate is concentrated under reduced pressure at 60°C to a water extract with a specific gravity of 1.10; water The extract was separated on an AB-8 column (aspect ratio of 1:15), and the loading volume was 1:4. First rinse with deionized water until the effluent is brown-yellow, then use 30% ethanol to rinse for 4 times the column volume, collect the 30% ethanol eluate, concentrate under reduced pressure to a specific gravity of 1.06 to obtain a preliminary separation concentrate; The concentrated solution was refined on an MDS column (diameter-to-height ratio: 1:12), rinsed with deionized water until the eluate was pale yellow, then rinsed with 30% ethanol for 4 column volumes, and collected 30% ethanol. lotion. Thin-layer chromatography detection (developing solvent is methyl ethyl ketone: ethyl ...

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Abstract

A process for preparing forsythiaside A comprises using forsythia shell as raw material, using deionized water and water alcohol as extracting solution, passing through preliminary separation column and refining column, using deionized water and water alcohol as eluent, and then obtaining forsythiaside A after being frozen and dried, the content can reaches 85%-99%. The invention uses cheap material, no toxic and safe solution, and has simple technology, high forsythia usage and low cost, which is suitable to the industrialized production, and the extracting rate is 0.5%-2%.

Description

Technical field: [0001] The invention relates to a method for extracting active ingredients from medicinal plants, in particular to a method for preparing forsythiaside A. Background technique: [0002] Forsythia is a deciduous shrub of Oleaceae Forsythia. The fruit is used as medicine, which has the functions of clearing away heat and detoxification, dispelling stagnation and swelling, antibacterial and inhibiting fungi. Forsythiaside A is its main component. It not only has a strong inhibitory effect on eleven kinds of pathogenic bacteria such as Staphylococcus aureus, but also has the effect of inhibiting phosphodiesterase and fungi. Recently, we found that it has a strong inhibitory effect on active oxygen. In view of its various biologically active functions, forsythiaside A has broad application prospects in the fields of medicine and food as natural medicines, natural preservatives and natural antioxidants. So far, there has been no report about the large amount of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H13/04A61K36/634
Inventor 张立伟霍丽丽王引珍
Owner SHANXI UNIV
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