Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

MS gene order of diffuse large B cell lymphoma related antigen specific TCR V alpha 6 subfamily and uses thereof

A technology of gene sequence and B cells, applied in the field of nucleotide sequence of T cell receptors, can solve problems such as antigen understanding

Inactive Publication Date: 2008-07-23
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT) +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on lymphoma at home and abroad mainly focuses on T-cell lymphoma, while there are few research materials on B-lymphocyte lymphoma.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • MS gene order of diffuse large B cell lymphoma related antigen specific TCR V alpha 6 subfamily and uses thereof
  • MS gene order of diffuse large B cell lymphoma related antigen specific TCR V alpha 6 subfamily and uses thereof
  • MS gene order of diffuse large B cell lymphoma related antigen specific TCR V alpha 6 subfamily and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0042] (1) Analysis of antigen-specific TCR gene sequence

[0043] The method for analyzing the length and base sequence of CDR3 of TCR Vα gene profile is to analyze the length of CDR3 of 29 subfamilies of TCR Vα by using RT-PCR-gene scanning method. First, the peripheral blood of patients with DLBL was collected, and the peripheral blood mononuclear cells were separated by Ficoll stratification method, and 1*10 7 RNA was extracted from PBMCs (peripheral blood mononuclear cells), and cDNA was synthesized by RT-PCR. At the same time, the gene sequence of the TCRα chain was found in the NCBI gene bank, and the positions of the V and C regions of each subfamily in the genome were marked. Design corresponding 29 Vα-specific upstream primers based on the cDNA of 29 Vα subfamily genes, and set a Cα downstream primer in the Cα region, and use PCR technology to amplify the V-N-JC region generated by the TCR rearrangement of the 29 Vα subfamily genes in patients . As shown in Figure...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a nucleotide sequence and the application of an antigen-specific T cell receptor of the diffusive large B cell lymphoma. According to the RT-PCR genescan analysis, the TCR V Alpha 6 subfamilies in the peripheral blood of patients shows an obviously monoclonal state, and the V-N-J region, namely, the complementary determining region 3, where the PCR products conduct the sequence analysis of the specific TCR sequence, is a part recognizably bound to the antigenic specificity; the genescan analysis also indicates that the TCR of the clonal T cell is a specific TCR produced due to the stimulus of the DLBL associated antigens, and is the basis and key for performing DLBL molecular targeted adoptive immunotherapy by means of the TCR transgenic technique. The nucleotide sequence of the specific TCR plays a significant role in further carrying out lymphoma-specific cellular immunotherapy.

Description

technical field [0001] The invention relates to a T cell receptor (TCR) nucleotide sequence, in particular to the MS gene sequence and application of diffuse large B cell lymphoma (DLBL) related antigen-specific TCR Vα6 subfamily. Background technique [0002] In recent years, the incidence of non-Hodgkin's lymphoma (NHL) has continued to rise. It is estimated that NHL will account for 4.4% of aggressive malignancies and 4.8% of all cancer deaths in the early 21st century. Among them, diffuse large B-cell lymphoma (DLBL) is the most common NHL, accounting for about 30% to 40%, and elderly patients over 60 years old account for 70%. For more than 20 years, CHOP regimen (cyclophosphamide, doxorubicin, vincristine, prednisone) was mainly used as the main chemotherapy. However, it has toxic effects on some elderly people, and the long-term survival rate is low. Although the recent emergence of targeted immunotherapy such as rituximab (CD20 monoclonal...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/12C07K14/725A61K31/7088A61K48/00A61P37/02
Inventor 谭获李扬秋尹青松陈少华杨力建叶静梅
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com