RG gene sequence for diffusivity large B cell lymphoma correlation antigen specific TCR Vbeta13 subfamily and application thereof

A technology of gene sequence and B cells, applied in the field of nucleotide sequence of T cell receptors, can solve problems such as antigen understanding

Inactive Publication Date: 2008-08-06
JINAN UNIVERSITY +1
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  • Abstract
  • Description
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Problems solved by technology

At present, the research on lymphoma at home and abroad mainly focuses on T-cell lymphoma, while there are few research materials on B-lymphocyte lymphoma.

Method used

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  • RG gene sequence for diffusivity large B cell lymphoma correlation antigen specific TCR Vbeta13 subfamily and application thereof

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Experimental program
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Embodiment

[0034] (1) Analysis of antigen-specific TCR gene sequence

[0035] The method for analyzing the length and base sequence of CDR3 of the TCR Vβ gene spectrum is to use the RT-PCR-gene scanning method to analyze the length of CDR3 of the 24 subfamilies of TCR Vβ. First, the peripheral blood of patients with DLBL was collected, and the peripheral blood mononuclear cells were separated by Ficoll stratification method, and 1*10 7 RNA was extracted from PBMCs (peripheral blood mononuclear cells), and cDNA was synthesized by RT-PCR. At the same time, the gene sequence of the TCRβ chain was found in the NCBI gene bank, and the positions of the V and C regions of each subfamily in the genome were marked. According to the cDNA of 24 Vβ subfamily genes, corresponding 24 Vβ specific upstream primers were designed, and a Cβ downstream primer was set in the Cβ region, and the V-NDN- JC District. As shown in Figure 1, fluorescein Fam marks the Cβ region of the PCR products of each subfami...

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Abstract

The invention discloses a nucleotide sequence which aims at the specificity T cell receptor of relative antigen of diffuse large b-cell lymphoma and the application, TCR V beta 13 sub-family in peripheral blood of DLBL patients is scanned and analyzed to take obvious monoclone through RT-PCR gens, a V-NDN-J area of specificity TCR sequence for sequencing PCR products namely a complementary-determining region 3 is a portion which combines with antigen specificity recognition, which expresses specificity TCR which is produced by the stimulation of TCR of clonal T cell for relative antigen of DLBL. The nucleotide sequence is a base and a key for adoptive cellular immunotherapy of DLBL molecular target through TCR transgenic technology. The nucleotide sequence of the specificity TCR is an important base for further developing specific cell immunization therapy which aims at lymphoma.

Description

technical field [0001] The invention relates to a T cell receptor (TCR) nucleotide sequence, in particular to a diffuse large B cell lymphoma (DLBL) related antigen-specific RG gene sequence and application of the TCR Vβ13 subfamily. Background technique [0002] In recent years, the incidence of non-Hodgkin's lymphoma (NHL) has continued to rise. It is estimated that NHL will account for 4.4% of aggressive malignancies and 4.8% of all cancer deaths in the early 21st century. Among them, diffuse large B-cell lymphoma (DLBL) is the most common NHL, accounting for about 30% to 40%, and elderly patients over 60 years old account for 70%. For more than 20 years, CHOP regimen (cyclophosphamide, doxorubicin, vincristine, prednisone) was mainly used as the main chemotherapy. However, it has toxic effects on some elderly people, and the long-term survival rate is low. Although the recent emergence of targeted immunotherapy such as rituximab (CD20 monoclonal antibody) has achieved...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12N15/10C07K14/725A61K31/7088A61K48/00A61P37/02
Inventor 李扬秋谭获尹青松陈少华杨力建叶静梅吴秀丽
Owner JINAN UNIVERSITY
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