Process for preparing bupleurum total saponin
A technology of total saponins and bupleurum, which is applied in the field of medicine, can solve the problems of not being able to obtain saikosaponin extracts, unfavorable industrialization, and many impurities in the extracts, which are difficult to directly apply to macroporous resin adsorption columns, so as to improve extraction efficiency and purity , good repeatability and reliable process
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Embodiment 1
[0030] Add 1000 grams of Bupleurum medicinal material, add 5 times the amount of 40% ethanol solution with pH 8, heat and reflux to extract twice, each time for 1 hour. Filtrate, combine, concentrate the filtrate, control the proportion of alcohol in the Bupleurum extract to 10%, mix well, centrifuge, and pour out the supernatant. Add an appropriate amount of 90% ethanol solution to the precipitate, stir to fully dissolve the total saponins of Bupleurum, centrifuge, take out and keep the supernatant, repeat the dissolution once, combine the centrifuged supernatant twice, concentrate at 70°C until there is no alcohol smell, Add water to dilute to 3 times the weight of the original medicinal material, and apply the processed HPD300 macroporous adsorption resin column for sufficient adsorption. First wash with purified water until the effluent is colorless, then use 3 column volumes of 30% ethanol to elute impurities, and then use 7 column volumes of 50% ethanol to elute, collect...
Embodiment 2
[0033] Add 1000 grams of Bupleurum medicinal material, add 15 times the amount of 95% ethanol solution with pH 11, heat and reflux for extraction 4 times, each time for 4 hours. Filtrate, combine, concentrate the filtrate, control the proportion of alcohol in the Bupleurum extract to 40%, mix well, centrifuge, and pour out the supernatant. Add an appropriate amount of 90% ethanol solution to the precipitate, stir to fully dissolve the total saponins of Bupleurum, centrifuge, take out and keep the supernatant, repeat the dissolution once, combine the centrifuged supernatant twice, concentrate at 70°C until there is no alcohol smell, Add an appropriate amount of water to dilute, and apply the treated HPD100 macroporous adsorption resin column for sufficient adsorption. Wash with purified water until the effluent is colorless, then elute impurities with 1 column volume of 60% ethanol, and then use 3 column volumes of 90% ethanol, collect the 90% ethanol eluate, and concentrate to...
Embodiment 3
[0036] 1000 grams of Bupleurum medicinal material, add 5 times the amount of 55% ethanol solution with pH 8, heat and reflux for extraction twice, each time for 1 hour. Filtrate, combine, concentrate the filtrate, control the proportion of alcohol in the Bupleurum extract to 10%, mix well, centrifuge, and pour out the supernatant. Add an appropriate amount of 90% ethanol solution to the precipitate, stir to fully dissolve the total saponins of Bupleurum, centrifuge, take out and keep the supernatant, repeat the dissolution once, combine the centrifuged supernatant twice, concentrate at 70°C until there is no alcohol smell, Add water to dilute to 3 times the weight of the original medicinal material, and put it on the D201 macroporous adsorption resin column that has been treated, and fully absorb it. First wash with purified water until the effluent is colorless, then use 3 column volumes of 30% ethanol to elute impurities, and then use 5 column volumes of 60% ethanol to elute...
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