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PCR detection method of transgene paddy strain Bt Shanyou 63

A technology of transgenic rice and detection method, applied in the field of flanking sequences of exogenous insert fragments

Inactive Publication Date: 2011-07-20
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the analysis of existing patents and documents, it is found that there are no articles or patent reports on the flanking sequence of the foreign insert of the transgenic rice line Bt Shanyou 63

Method used

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  • PCR detection method of transgene paddy strain Bt Shanyou 63
  • PCR detection method of transgene paddy strain Bt Shanyou 63
  • PCR detection method of transgene paddy strain Bt Shanyou 63

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Cloning of the Side Sequence of the Foreign Insertion Vector of the Transgenic Rice Line Bt Shanyou 63

[0032] 1. Experimental materials

[0033] 1. Plant material

[0034]Transgenic rice: Transgenic rice line Bt Shanyou 63.

[0035] Conventional rice: Shanyou 63.

[0036] 2. Enzymes and reagents

[0037] Molecular biology reagents, such as dNTPs, Taq DNA polymerase, and DL2000 Marker were purchased from Dalian Bao Biological Engineering Co., Ltd. Other biochemical reagents are imported subpackages or domestic analytical pure. Primers and degenerate primers were synthesized by Beijing Aoke Biotechnology Co., Ltd.

[0038] 3. Experimental equipment

[0039] PCR instrument: Eppendorf Mastercycle gradient (Eppendorf co.)

[0040] Nucleic acid electrophoresis instrument: DYY-III nucleic acid electrophoresis instrument (Beijing Liuyi Instrument Factory)

[0041] DNA sequencer (Applied Biosystem377 automatic fluorescent sequence analyzer)

[0042] DNA electrophoresi...

Embodiment 2

[0141] Qualitative PCR detection method based on the flanking sequence of the exogenous insertion vector of the transgenic rice line Bt Shanyou 63

[0142] 1. Experimental materials

[0143] 1. Plant material

[0144] Transgenic rice: Transgenic rice line Bt Shanyou 63.

[0145] Conventional rice: Shanyou 63.

[0146] 2. Enzymes and reagents

[0147] Molecular biology reagents, such as dNTPs, Taq DNA polymerase, and DL2000 Marker were purchased from Dalian Bao Biological Engineering Co., Ltd.

[0148] Other biochemical reagents are imported subpackages or domestic analytical pure. Primers were synthesized by Beijing Aoke Biotechnology Co., Ltd.

[0149] 3. Experimental equipment

[0150] PCR instrument: Eppendorf Mastercycle gradient (Eppendorf co.)

[0151] Nucleic acid electrophoresis instrument: DYY-III nucleic acid electrophoresis instrument (Beijing Liuyi Instrument Factory)

[0152] DNA electrophoresis analysis system: Kodak EDAS 290 (Kodak co.)

[0153] Other i...

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Abstract

The invention relates to a side sequence of exogenous interpose fragments of transgene paddy rice line Bt SHANYOU 63, its 5' side sequence is shown as SEQ ID NO1 and its 3' side sequence is shown as SEQ ID NO2. The line specificity determine the nature and definite quantity PCR detection of sensitive idiosyncratic transgene paddy rice line Bt SHANYOU 63 is constructed by using side sequence as aim DNA amplification fragments.

Description

technical field [0001] The present invention relates to a gene sequence in the field of biotechnology. Specifically, it relates to a flanking sequence of an exogenous insertion fragment of a transgenic rice line Bt Shanyou 63. Background technique [0002] Rice is one of the most important food crops in my country. With the extensive research and application of transgenic technology in rice, several transgenic rice strains have been released to the environment and applied for commercial planting. Strain-specific detection of transgenic plants is an important technical basis for the supervision and management of transgenic plants to ensure their healthy development. The flanking sequence of the exogenous insert in a transgenic plant is one of the most important molecular characteristics of a transgenic plant line. Therefore, the flanking sequence of the exogenous insert is an important technical data for establishing a specific detection method for a transgenic plant line. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12Q1/68
Inventor 谢家建王锡锋彭于发
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI