Method of mixed fermentation treatment using vinasse as main feed raw material
A technology of mixing fermentation and feed raw materials, applied in the direction of animal feed, animal feed, application, etc., can solve problems such as environmental pollution of distiller's grains, achieve the effect of good palatability and increase total protein content
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Embodiment 1
[0051] Mixed fermentation process using distiller's grains as the main feed raw material, one of the strains used is Brevibacterium lactofermentum, its preservation number is CICC 10227, and the second strain is Candida tails , whose preservation number is CICC 1314, and the strains are preserved on the slant of the culture medium.
[0052] The Brevibacterium lactis fermentum and Candida utilis were respectively cultured by three-stage seeds, mixed and inoculated in the mixed seed medium to obtain the mixed seed liquid of the Brevibacterium lactis fermentum and Candida utilis, and then the mixed seeds The inoculum solution was inoculated in a mixed fermentation medium with a humidity of 50% at an inoculation amount of 1:10. After the inoculated mixture was fermented in stages at a temperature of 30-37°C, the fermented product was then placed in a 50°C refrigerator. After drying at high temperature and packaging, the finished product is obtained.
[0053] The tertiary seed cul...
Embodiment 2
[0071] Brevibacterium lactis fermentum and Candida utilis were respectively subjected to three-stage seed culture, and the three-stage seed liquid was mixed and inoculated in the mixed seed medium to obtain a mixed seed liquid of Brevibacterium lactis fermentum and Candida utilis, and then The mixed seed solution is inoculated in a mixed fermentation medium with a humidity of 60% according to the inoculum amount of 1:10, and the inoculated mixture is fermented in stages at a temperature of 30-37°C, and the fermented product obtained is then placed After drying and packaging at a temperature of 40°C, the finished product is obtained.
[0072] The culture process of the mixed seed liquid of described fermenting Brevibacterium lactis and Candida utilis is:
[0073] Brevibacterium lactis fermentum and Candida utilis were mixed according to the ratio of 1:1, and then inoculated into the seed medium according to the inoculum size of 1:10, the culture temperature was 28°C, the stirri...
Embodiment 3
[0079] Brevibacterium lactis fermentum and Candida utilis were respectively subjected to three-stage seed culture, and the three-stage seed liquid was mixed and inoculated in the mixed seed medium to obtain a mixed seed liquid of Brevibacterium lactis fermentum and Candida utilis, and then The mixed seed solution is inoculated in the mixed fermentation medium with a humidity of 55% according to the inoculum amount of 1:10, and the inoculated mixture is fermented in stages at a temperature of 30-37°C, and the obtained fermented product is placed in the The finished product is obtained after drying and packaging at a temperature of 60°C.
[0080] The culture process of the mixed seed liquid of described fermenting Brevibacterium lactis and Candida utilis is:
[0081]Brevibacterium lactis fermentum and Candida utilis were mixed according to the ratio of 1:0.01, and then inoculated into the seed medium according to the inoculum size of 1:10, the culture temperature was 30°C, the s...
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