Method for detecting enoxacin in biological body fluid with Mn doping ZnS quantum point room temperature phosphorescent

A technology of enoxacin and room temperature phosphorescence, which is applied in the fields of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of derivatizer toxicity, expensive equipment, cumbersome operation, etc., to avoid sample pretreatment process, selectivity enhancement, avoid interference effect

Inactive Publication Date: 2008-10-08
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the methods commonly used to separate and detect enoxacin in complex samples are various combined techniques, but they need to derivatize the analyte, the operation is cumbersome, and the derivatizers use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1, room temperature phosphorescence detection of enoxacin in biological fluid

[0020] 1. Preparation of Mn-doped ZnS quantum dots:

[0021] 1mmol of L-cysteine, 0.5mmol of ZnSO 4 and 0.015mmol of MnCl 2 Add it to 45ml of water, adjust the pH value of the solution to 11 with NaOH, protect with argon, stir at room temperature for 30min, and dissolve 5ml of 0.1mol / L Na 2 S was quickly injected into the solution, and continued to stir at room temperature for 30 minutes, and then the solution was heated to 50°C in air and aged for 2 hours, purified, and vacuum-dried to obtain Mn-doped ZnS quantum dot products.

[0022] 2. Preparation of Mn-doped ZnS quantum dot mother liquor:

[0023] Weigh 50mg of Mn-doped ZnS quantum dots, and set the volume in a 100ml volumetric flask.

[0024] 3. Handling of actual samples:

[0025] Urine samples are routinely diluted 80-fold without further complicated sample pretreatment.

[0026] 4. Detection of enoxacin in urine sample...

Embodiment 2

[0028] Example 2, room temperature phosphorescence detection of enoxacin in biological fluid

[0029] 1. Preparation of Mn-doped ZnS quantum dots:

[0030] 2mmol of L-cysteine, 1mmol of ZnSO 4 and 0.03mmol of MnCl 2 Add it to 90ml of water, adjust the pH value of the solution to 11 with NaOH, protect it with argon, stir at room temperature for 30min, and dissolve 10ml of 0.1mol / L Na 2 S was quickly injected into the solution, and continued to stir at room temperature for 30 minutes, and then the solution was heated to 50°C in air and aged for 2 hours, purified, and vacuum-dried to obtain Mn-doped ZnS quantum dot products.

[0031] 2. Preparation of Mn-doped ZnS quantum dot mother liquor:

[0032] Weigh 50mg of Mn-doped ZnS quantum dots and dissolve in a 100mL volumetric flask.

[0033] 3. Handling of actual samples:

[0034] Serum samples are routinely diluted 50 times without further complicated sample pretreatment process.

[0035] 4. Detection of enoxacin in serum sam...

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PUM

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Abstract

The invention relates to a method of a Mn doped ZnS quantum dots room temperature phosphorescence detecting an enoxacin in the biological body fluids. The invention uses the room temperature phosphorescence properties of the Mn doped ZnS quantum dots, and provides a simple, fast, economical, keen and highly selective method to detect the enoxacin in the biology body fluids; the Mn doped ZnS quantum dots are dissolved to be a solution, a urine sample or a serum sample is diluted into 30, 50 or 80 times for detecting the linear equation of the enoxacin, whose linear range is 0.2-7.2 mu mol/L, and detection limit is 58.6 nmol/L; when the method is used to detect the enoxacin in the biological body fluids, an oxygen scavenger and a inducer are not need to add, and the background fluorescence and the interference of scattered light can be avoided. Simultaneously, due to the high selectivity of the method, when the enoxacin is detected in the biological body fluids, a complex sample pretreatment process is not needed.

Description

【Technical field】: [0001] The invention relates to aqueous phase synthesis of Mn-doped ZnS quantum dots for room-temperature phosphorescence detection of enoxacin in biological fluids, and belongs to the technical field of biological analysis and detection. 【Background technique】: [0002] Quantum dots are mainly semiconductor nanoparticles composed of II-VI group elements or III-V group elements. Compared with organic fluorescent dyes, the photoluminescent properties of quantum dots are very superior: long-range excitation, narrow and symmetrical emission peaks, large Stokes shift, high quantum yield, and difficult photolysis. The fluorescent properties of quantum dots have been widely used to detect various ions, small molecules and biological macromolecules. However, the phosphorescent properties of quantum dots and their applications in analytical detection have received less attention. [0003] Room temperature phosphorescence has many advantages over fluorescence ana...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 严秀平何瑜李妍
Owner NANKAI UNIV
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