Use of fungal mutants for expression of antibodies

Abstract

The present invention relates to a filamentous fungal cell in which an endogenous alkaline protease activity, an endogenous neutral metalloprotease activity and an endogenous serine protease activity of the subtilisin type have been completely or partially inactivated. Particularly the endogenous alkaline protease activity, the endogenous neutral metalloprotease activity and the endogenous serine protease activity of the subtilisin type are encoded by the alp, npI and pepC genes respectively. The filamentous fungal cell is particularly suitable for production of heterologous proteins such as antibodies.

Description

Use of fungal mutants for expressing antibodies technical field The present invention relates to filamentous fungal cells wherein the activities of endogenous alkaline proteases, endogenous metalloproteases and endogenous serine proteases are all or partially inactivated. Furthermore, the present invention relates to methods of producing heterologous polypeptides in filamentous fungal host cells. Background technique In recent years, the use of recombinant host cells in the expression of heterologous proteins has greatly simplified the production of large quantities of commercially valuable proteins that could otherwise only be obtained by purification from their natural sources. Currently, there are many choices of expression systems from which to choose for the production of any given protein, including bacterial and eukaryotic hosts. The choice of an appropriate expression system often depends not only on the ability of the host cell to produce sufficient quantities of...

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