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Stabilized liquid enzyme compositions

一种液体组合物、肽化合物的技术，应用在洗涤剂组合物、酶稳定、酶等方向，能够解决枯草杆菌蛋白酶和其他酶稳定性降低、液体洗涤剂洗涤性能降低等问题

Active Publication Date: 2011-02-23
NOVOZYMES AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, the stability of subtilisin and other enzymes in the liquid detergent composition is reduced, resulting in reduced wash performance of the liquid detergent

Method used

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  • Stabilized liquid enzyme compositions
  • Stabilized liquid enzyme compositions
  • Stabilized liquid enzyme compositions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] The different peptide aldehydes were produced by custom peptide synthesis companies, all with >80% purity. Peptide aldehydes were dissolved in DMSO to a concentration of 10 mg / ml before use.

[0065] Prepare model liquid diluents for testing different stabilizers:

[0066] Detergent base:

[0067] components

%w / w

Sodium Alkyl Ethoxy Sulfate (C9-15, 2EO)

6.0

Sodium dodecyl benzene sulfonate

3.0

Sodium toluenesulfonate

3.0

Oleic acid

2.0

Primary Alcohol Ethoxylates (C12-15, 7EO)

3.0

Primary Alcohol Ethoxylates (C12-15, 3EO)

2.5

ethanol

0.5

Monopropylene Glycol

2.0

Trisodium Citrate Dihydrate

4.0

Triethanolamine

0.4

Deionized water

add up to 100%

[0068] pH adjusted to 8.5 with NaOH

[0069] A control detergent with enzyme was prepared:

[0070] Detergent A:

[0071] components

%w / w

...

Embodiment 2

[0078] The following control detergents with enzymes were prepared:

[0079] Detergent L:

[0080] components

%w / w

Detergent base from Example 1

98.5

Protease (Savinase 16.0LEX)

0.5

Lipase (Lipex 100L)

0.5

Amylase (Stainzyme 12L)

0.5

[0081] The following detergents with stabilizers according to the invention were prepared and standardized to 100 g of detergents:

[0082]

[0083] The detergents were placed in closed glasses at 25°C and 35°C. Residual lipase, amylase and protease activities were measured at different times (w = weeks) (by comparison with controls stored at -18°C) using standard enzymatic assays (by hydrolysis of N,N -Dimethylcasein to measure protease, lipase by hydrolysis of p-nitrophenyl valerate at 40°C, pH 7.7, and 4,6-ethylene-( G7) p-nitrophenyl-(G1)-α, D-maltoheptanoside (4,6-ethylene-(G7)p-nitrophenyl-(G1)-α, D-maltoheptasoid) to measure amylase).

[0084]

[0085] It can be...

Embodiment 3

[0087] Peptide aldehydes Z-GAF-H, Z-GAL-H and Z-GAY-H were produced by peptide synthesis, all with >80% purity. Peptide aldehydes were dissolved in DMSO to a concentration of 10 mg / ml before use.

[0088] The following detergent N with enzymes was prepared:

[0089] components

%w / w

Sodium Alkyl Ethoxy Sulfate (C9-15, 2EO)

20.0

Sodium toluenesulfonate

3.0

Oleic acid

4.0

Primary Alcohol Ethoxylates (C12-15, 7EO)

2.5

Primary Alcohol Ethoxylates (C12-15, 3EO)

2.0

ethanol

2.1

Sodium carbonate

4.5

Trisodium Citrate Dihydrate

5.0

Deionized water

Add to 99%

pH adjusted to 8.0 with NaOH

Protease (Savinase 16.0LEX)

0.5

Lipase (Lipex 100L)

0.5

[0090]The following detergents with stabilizers according to the invention were prepared and standardized to 100 g of detergents:

[0091]

[0092] The detergent was placed in a clos...

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PUM

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Abstract

Peptide aldehydes or ketone derivatives are particularly efficient for stabilizing subtilisin-type proteases in aqueous compositions such as liquid detergents, including peptide compounds with OH-substituted phenylalanine aldehyde as the C-terminal residue.

Description

field of invention [0001] The present invention relates to a liquid composition comprising subtilisin and a peptide compound as a stabilizer for the subtilisin. The present invention also relates to peptide compounds useful as stabilizers for subtilisins. Background of the invention [0002] Proteases of the subtilisin type are well known in the field of liquid aqueous detergents, in particular their use in laundry washing. A problem commonly faced in such liquid detergents is the degradation of other enzymes in the composition by subtilisin as well as subtilisin itself. As a result, the stability of subtilisin and other enzymes in the liquid detergent composition is reduced, resulting in reduced wash performance of the liquid detergent. [0003] The prior art is mainly devoted to improving the storage stability of enzymes in liquid detergents, for example by adding different subtilisin inhibitors or stabilizers. Boronic acid and boronic acid are known to reversibly inhib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/08C11D3/386C07K2/00C07K5/10
CPCC07K5/0808C07K5/0806C07K2/00C07K5/101C07K5/1016C07K5/0817C07K5/1024C11D3/38663C12N9/54C12N9/96C12Y304/21062Y02P20/55
Inventor 洛恩·K·尼尔森利斯·M·米克尔森艾斯本·P·弗里伊斯于尔根·C·F·诺伊策尔奥勒·西蒙森洛特·R·索伦森
Owner NOVOZYMES AS
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