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Erythrocyte immune extracorporeal Chinese medicine sensitivity experimental method

An experimental method and red blood cell technology are applied in the field of in vitro drug susceptibility experiments of traditional Chinese medicines to achieve the effects of true and reliable detection results, easy popularization and promotion, and accurate and reliable detection results.

Inactive Publication Date: 2008-10-29
常国良
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0007] There is no report on the application of red blood cell immune function detection method to the in vitro drug sensitivity test of traditional Chinese medicine and the study of its immunological principle

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Preparation of cell suspension

[0028] Take 1-2mL of fasting venous blood from the subjects, anticoagulate with heparin, equilibrate with normal saline, centrifuge at 2500r / min for 5min, extract plasma into a test tube, and mark it for later use.

[0029] Inject about 4mL of normal saline into another test tube, extract 10uL of whole blood cells from the middle layer of the separated whole blood cells, add it into the normal saline test tube, pipette evenly, count red blood cells under a microscope, and prepare a red blood cell count of 1.25×10 7 / mL of whole blood cell suspension and marked for later use.

[0030] 2. Preparation of yeast solution

[0031] 1.0×10 8 The / mL yeast solution was provided by the Blood Immunology Laboratory of Changhai Hospital, Second Military Medical University, Shanghai.

[0032] Cut the frozen yeast solution, thaw it at room temperature, blow it until it is uniform, and then use it.

[0033] 3. Blank control group without interven...

Embodiment 2

[0042] For cerebral infarction cases, Western medicine generally classifies them according to the infarction site, size and responsible blood vessel. Traditional Chinese medicine uses syndrome differentiation, which is generally divided into qi deficiency and blood stasis syndrome, phlegm turbidity and blood stasis syndrome, and qi and yin deficiency syndrome. Different types of syndrome differentiation, its treatment and medication are different. normally:

[0043] For qi deficiency and blood stasis syndrome, the treatment should be benefiting qi and activating blood circulation, medicinal Astragalus injection + Danshen injection;

[0044] For phlegm turbidity and blood stasis syndrome, it should be treated by resolving phlegm and removing blood stasis, refreshing the brain and resuscitation, medicinal compound musk injection;

[0045] Qi and Yin Deficiency Syndrome, the treatment should benefit Qi and nourish Yin, promote blood circulation and remove blood stasis, medicina...

Embodiment 3

[0064] For patients with asthma, Western medicine generally divides it into pulmonary and cardiogenic. Traditional Chinese medicine, on the other hand, is based on syndrome differentiation and classification, which can be divided into syndromes of lung qi deficiency, syndromes of phlegm and heat obstructing the lungs, and syndromes of water lingering in the heart and lungs. Different types of syndrome differentiation, its treatment and medication are different.

[0065] Lung qi deficiency syndrome, treatment should benefit qi and relieve asthma, medicinal astragalus injection;

[0066] For the syndrome of phlegm-heat obstructing the lung, it should be treated by clearing the lung and resolving phlegm, and medicinal Tanreqing injection;

[0067] For the syndrome of Shui Ling heart and lung, it should be treated by warming yang and diuresis, and medicinal Shenfu injection.

[0068] However, there are many subjective factors in TCM syndrome differentiation, and it is often diff...

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Abstract

The invention relates to a method for red blood cell immune exoteric traditional Chinese medicine sensitivity test; the method comprises the following steps: a red blood cell immune yeast rosette test is adopted as the basis; the traditional Chinese medicine is adopted as interfere factor; the detection for the red blood cell immune index RBC-C3bRR of a drug interfere group added with the traditional Chinese medicine, a blank control group without interference and a test control group interfered by physiological saline is carried out; the sensitivity of the traditional Chinese medicine is determined according to the detected result; if the traditional Chinese medicine occurs up-regulation to the RBC-C3bRR, the traditional Chinese medicine is sensitive and effective drug; if the traditional Chinese medicine occurs down-regulation or does not occur regulation effect, the traditional Chinese medicine is insensitive or ineffective drug; the test method of the invention has the effect of syndrome differentiation through drug effect outside of the body on syndrome differentiation treatment of traditional Chinese medicine, and objectifies and indexes the syndrome differentiation treatment of traditional Chinese medicine, thus avoiding the subjectivity and the randomness of the application of the traditional Chinese medicine.

Description

technical field [0001] The invention relates to a red blood cell immune function detection technology, in particular to a method for using the red blood cell immune function detection method to conduct a drug sensitivity test. The invention uses the red blood cell immune function to conduct an in vitro traditional Chinese medicine drug sensitivity test for the first time. Background technique [0002] Since the red blood cell was discovered in 1658, it has been considered as a transporter of oxygen and carbon dioxide. With the advancement of science and technology, people gradually realized more functions of red blood cells. In 1981, American reproductive immunologist Siegel first proposed the new concept of "red blood cell immune system" on the basis of previous research, which updated people's understanding of red blood cell function. With the heating up of innate immunity research, in 1990, Paccaud conducted a comparative study on the complement receptors of erythrocyte ...

Claims

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Application Information

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IPC IPC(8): G01N33/556G01N33/15
Inventor 常国良
Owner 常国良
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