PiggyBac used as genetic operation and analysis tool of vertebrate

A technology for vertebrates and species, applied to cells modified by the introduction of foreign genetic material, biochemical equipment and methods, and the use of vectors to introduce foreign genetic material, etc., can solve the problems of limited number of transposon systems and limitations
CN101297031AActive Publication Date: 2008-10-29FUDAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
FUDAN UNIV
Publication Date
2008-10-29

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Abstract

The present invention relates to transgenic vertebrate, including mammalian, cells, whose genomes comprise one or more elements of the piggyBac family transposon system. Transgenic non-human vertebrates, including transgenic non-human mammals, whose genomes comprise one or more elements of the piggyBac family transposon system, are also provided. Methods of making and using the cells and animals of the invention, including applications in the medical, veterinary, and agricultural fields, are additionally provided. The present invention also relates to kits useful for practicing such methods.
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Description

1. Field of invention

[0001] The present invention relates to transgenic vertebrate (including mammalian) cells and transgenic non-human vertebrate cells, including non-human mammals, the genome of which comprises one or more elements of the piggyBac family transposon system, and the preparation and use of said cells and animal approach. The invention also relates to kits that can be used to practice these methods. 2. Background of the invention

[0002] Transposable elements, or transposons, are mobile genetic units identified in many metazoans, including worms, insects, and humans. In humans and mice, sequences derived from transposons account for more than 40% of the genome (Lander et al., 2001, Nature 409: 860-921; Waterston et al., 2002, Nature 420: 520-562), indicating that transposition is importance in evolution. Since the discovery of the first transposon in maize by McClintock (McClintock, 1950, Proc. Nat'l. Acad. Sci. USA 36:344-345), transposable elements have...

Claims

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