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PiggyBac used as genetic operation and analysis tool of vertebrate

A technology for vertebrates and species, applied to cells modified by the introduction of foreign genetic material, biochemical equipment and methods, and the use of vectors to introduce foreign genetic material, etc., can solve the problems of limited number of transposon systems and limitations

Active Publication Date: 2008-10-29
FUDAN UNIV
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Problems solved by technology

Although the limitation to dipteran vectors is due in part to the limited number of transposon systems obtained from non-dipteran species, given that functional transposons can have detrimental effects on the host genome, there is little concern for systems with transposon function. Limitations occur not unexpectedly

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  • PiggyBac used as genetic operation and analysis tool of vertebrate
  • PiggyBac used as genetic operation and analysis tool of vertebrate
  • PiggyBac used as genetic operation and analysis tool of vertebrate

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Embodiment Construction

[0066] The present invention provides the use of piggyBac-like transposon systems in vertebrate cells and non-human vertebrate organisms. The invention provides vertebrate cells and non-human organisms engineered to express components of the piggyBac-like transposon system, methods of making such cells and organisms, and libraries of such engineered cells and organisms.

[0067] The present invention relates to the introduction of the piggyBac-like transposon of the present invention into the genome of a cell. Efficient incorporation of transposons occurs when cells also contain a piggyBac-like transposase. As described above, the piggyBac-like transposase can be provided to the cell as a piggyBac-like transposase protein or as a nucleic acid encoding the piggyBac-like transposase. The nucleic acid encoding the piggyBac-like transposase can be in the form of RNA or DNA. In addition, nucleic acids encoding piggyBac-like transposases can be stably or transiently incorporated i...

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Abstract

The present invention relates to transgenic vertebrate, including mammalian, cells, whose genomes comprise one or more elements of the piggyBac family transposon system. Transgenic non-human vertebrates, including transgenic non-human mammals, whose genomes comprise one or more elements of the piggyBac family transposon system, are also provided. Methods of making and using the cells and animals of the invention, including applications in the medical, veterinary, and agricultural fields, are additionally provided. The present invention also relates to kits useful for practicing such methods.

Description

1. Field of invention [0001] The present invention relates to transgenic vertebrate (including mammalian) cells and transgenic non-human vertebrate cells, including non-human mammals, the genome of which comprises one or more elements of the piggyBac family transposon system, and the preparation and use of said cells and animal approach. The invention also relates to kits that can be used to practice these methods. 2. Background of the invention [0002] Transposable elements, or transposons, are mobile genetic units identified in many metazoans, including worms, insects, and humans. In humans and mice, sequences derived from transposons account for more than 40% of the genome (Lander et al., 2001, Nature 409: 860-921; Waterston et al., 2002, Nature 420: 520-562), indicating that transposition is importance in evolution. Since the discovery of the first transposon in maize by McClintock (McClintock, 1950, Proc. Nat'l. Acad. Sci. USA 36:344-345), transposable elements have...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/22C12N15/85C12N5/16C12N5/22C12N15/09C12N15/86
CPCC12N15/8509C12N2800/90A01K2227/105A01K2217/05A01K67/0275A01K2267/03
Inventor T·徐M·韩Y·庄X·吴S·丁G·李
Owner FUDAN UNIV
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