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Method for detecting erythromycin series compounds and special-purpose enzyme-linked immunologic reagent kit thereof

An enzyme-linked immunosorbent reagent and erythromycin technology, which is applied to cells modified by introducing foreign genetic material, measuring devices, fusion cells, etc., can solve the problem that the instrument method is not suitable for on-site monitoring and screening of a large number of samples, and the complex operation of instruments and equipment Process, cumbersome and other problems, to achieve the effect of shortening the detection time, considerable social and economic benefits, and simplifying the steps

Inactive Publication Date: 2012-05-30
贵州勤邦食品安全科学技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The chemical methods for detecting erythromycin residues are mainly instrumental methods such as gas chromatography and gas chromatography-mass spectrometry. Due to the complexity of the equipment and the cumbersome operation process, the instrumental methods are not suitable for on-site monitoring and screening of a large number of samples.

Method used

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  • Method for detecting erythromycin series compounds and special-purpose enzyme-linked immunologic reagent kit thereof
  • Method for detecting erythromycin series compounds and special-purpose enzyme-linked immunologic reagent kit thereof
  • Method for detecting erythromycin series compounds and special-purpose enzyme-linked immunologic reagent kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Kit and detection method using erythromycin hapten and carrier protein conjugate as coating source

[0028] 1. The detection principle of the kit with erythromycin hapten and carrier protein conjugate as the coating source is as follows:

[0029] When the erythromycin-conjugated antigen is pre-coated on the microwell strip of the microtiter plate, after adding the sample solution or standard solution, the enzyme-labeled secondary antibody is added immediately, and then the erythromycin-specific antibody solution is added. The erythromycin drug competes with the erythromycin-coupled antigen coated on the ELISA plate for the erythromycin-specific antibody, and the color is developed with a chromogenic solution. The absorbance value of the sample is negatively correlated with the content of the erythromycin drug, and the standard curve By comparison, the residual amount of erythromycin in the sample can be obtained. At the same time, the concentration range of ...

Embodiment 2

[0094] Embodiment 2, test kit precision, accuracy and shelf life test

[0095] 1. Standard product precision test:

[0096] From the three different batches of kits prepared in step 3 of Example 1, 10 kits were respectively extracted, and 20 microwells were respectively extracted from the enzyme-linked plate of each kit, and measured Calculate the coefficient of variation from the absorbance value of the 1.8 μg / L erythromycin standard solution. The results are shown in Table 1.

[0097] Table 1. Standard repeatability test (CV%)

[0098]

[0099] Experiments show that the coefficients of variation of standard products measured 10 times by each batch of kits are all between 3.5% and 12.6%, which meets the requirement that the precision is less than or equal to 20%.

[0100] 2. Sample precision and accuracy test

[0101] (1) Sample precision test:

[0102] Add erythromycin with a final concentration of 40 μg / L to chicken, chicken liver, pork or pork liver without erythro...

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Abstract

The present invention discloses a method for detecting the erythromycin compounds and an enzyme linked immunoreaction reagent kit thereof which is specialized for the method. The enzyme linked immunoreaction reagent kit comprises an erythromycin specific antibody, a coatingen and an enzyme label. The erythromycin specific antibody is a monoclone antibody or polyclonal antibody of the erythromycin. The erythromycin compound is at least one in erythromycin, erythromycin thiocyanate and erythromycin ethylsuccinate. The erythromycin is erythromycin A. The enzyme linked immunoreaction reagent kit of the invention has the advantages of simple structure, convenient use, low cost, convenient carriage, high efficiency of the detecting method, accuracy and easiness. The on-spot monitoring can be executed. Furthermore the enzyme linked immunoreaction reagent kit is suitable for the qualitative and quantitative sieving of considerable samples, and can exert an important function in the detection to the erythromycin.

Description

technical field [0001] The invention relates to a method for detecting erythromycin compounds and a special ELISA kit. Background technique [0002] Erythromycin compounds are macrolide antibiotics, mainly used for anti-bacteria and mycoplasma infection in livestock and poultry, and can also be used as feed additives for pigs to promote weight gain and improve feed conversion rate. But erythromycin compounds can produce some side effects on the body, such as gastrointestinal reactions, cholestatic jaundice and phlebitis caused by intravenous infusion, and can also cause necrotic liver disease, mental disorders and arthritis syndrome. Therefore, The extensive application of erythromycin compounds in livestock and poultry production will lead to their residues in livestock and poultry products, endangering public health. At present, the No. 235 document of the Ministry of Agriculture has stipulated that the maximum residue limit of this drug is 200μg / kg. [0003] The chemica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/543G01N33/535C12N5/12
Inventor 沈建忠何方洋万宇平冯才伟冯才茂汪善良赵正苗刘玉梅罗晓琴
Owner 贵州勤邦食品安全科学技术有限公司
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