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Tissue culture method for Beijing poplar

A tissue culture, culture medium technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., to achieve the effect of high reproduction rate

Inactive Publication Date: 2011-07-06
HUNAN MAOYUAN FORESTRY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because of its good material, fast growth, and strong resistance, it is very popular among the people, but for many years it has been mainly used for conventional cutting seedlings, and its reproduction rate is low, which is far from meeting the needs of the society.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0017] 1 Materials and methods

[0018] 1.1 Test material

[0019] Beijing Yang

[0020] 1.2 Test method

[0021] 1.2.1 Material Handling

[0022] Freshly sprouted leaves of indoor potted seedlings, cut off the petioles, rinsed repeatedly with sterile water for 30 minutes, and blotted the water with filter paper for later use.

[0023] 1.2.2 Induction and differentiation culture of primary buds

[0024] Place the explants on an ultra-clean workbench, sterilize with 0.1% mercuric chloride for 6 minutes, and rinse with sterile water for 5 times. The wounds at both ends of the petiole were excised, placed flat on the induction medium, and an explant was connected to each inoculated bottle, and placed on a culture rack for light cultivation.

[0025] 1.2.3 Stem pumping culture

[0026] Cut the induced clustered buds into small buds, and insert them into the stem-shooting culture basics added with different hormone concentrations for stem-shooting culture.

[0027] 1.2.4 Str...

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PUM

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Abstract

The invention discloses a populus X beijengensis tissue culturing method which is characterized by comprising the following steps: (1) material collection and initial medium sprout induction differentiation culture; (2) cespitose sprout stooling culture; (3) sound seedling culture; (4) enrichment culture; (5) rooting culture; (6) hardening-seedling and transplanting. The method conducts vitro culture to the populus X beijengensis and has the advantage of high reproduction rate.

Description

1. Technical field: [0001] The invention relates to a seedling cultivation technique, in particular to a poplar tissue cultivation method. 2. Background technology: [0002] Beijing poplar is a new variety of poplar selected and bred in my country, which is a hybrid offspring of Zhuantian poplar and Qingyang poplar. Its wood is soft and has long fibers, which can be used for construction, bridges, mine pillars, plywood, and papermaking. It is also an excellent timber forest, shelter forest, street tree and "surrounding" greening tree species. Because of its good material, fast growth, and strong resistance, it is very popular among the people. However, for many years, conventional cuttings have been the main method of raising seedlings, and the reproduction rate is low, which is far from meeting the needs of the society. 3. Contents of the invention: [0003] The purpose of the present invention is to provide a tissue culture technique for in vitro culture of Populus ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 唐作钧李重立李绍华梁丽容肖兴翠王丛亚
Owner HUNAN MAOYUAN FORESTRY CO LTD