Miniature Chinese rose tissue culture breeding method

A rose and miniature technology, applied in the field of plant cultivation, can solve the problems of high cost of cutting propagation, low survival rate, difficulty in mass production, etc., and achieve the effect of increasing the reproduction rate and survival rate

Active Publication Date: 2015-02-25
启东欣荣旅游开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Miniature roses can be propagated by seeds, cuttings and tissue culture techniques. Cuttings are often used for propagation, but the cost of cutting propagation is high, the survival rate is low, a

Method used

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Examples

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Example Embodiment

[0022] In order to deepen the understanding of the present invention, the present invention will be described in further detail below with reference to the examples, which are only used to explain the present invention and do not constitute a limitation on the protection scope of the present invention. Example 1:

[0023] Select robust stem segments of miniature roses that have been sterilized in advance as explants, after decontamination, sterilization and disinfection, the explants are cut into stem segments containing 2 intact scales, and the stem segments are immersed in the culture medium, wherein KT The content is 0.2mg / L, 6-BA content is 0.5mg / L, NAA content is 0.005mg / L, pH value is 5, 0.3% activated carbon is added, the culture temperature is adjusted to 25°C, and the light is illuminated at 1500Lux for 10h to induce induction. Stem segment with axillary buds.

[0024] The axillary buds were cut off and immersed in the medium, wherein the KT content was 0.2 mg / L, the...

Example Embodiment

[0027] Example 2:

[0028] Select robust stem segments of miniature roses that have been sterilized in advance as explants, after decontamination, sterilization and disinfection, the explants are cut into stem segments containing 1 complete scale leaf, and the stem segments are immersed in the culture medium, wherein KT The content was 0.2mg / L, the 6-BA content was 2mg / L, the NAA content was 0.005mg / L, the pH value was 6, 0.5% activated carbon was added, the culture temperature was adjusted to 25°C, and the stems were induced by light at 1200Lux intensity for 15h. Segmented axillary buds.

[0029] The axillary buds were cut and immersed in the medium, where the KT content was 0.2 mg / L, the 6-BA content was 0.5 mg / L, the NAA content was 0.005 mg / L, the pH value was 5, and the culture temperature was adjusted to 30 ° C, 1200 Lux Under the intensity of light for 15h, until the axillary buds grow to 2-3cm, normal seedlings are formed, and the seedlings are immersed in the rooting...

Example Embodiment

[0032] Example 3:

[0033] Select robust stem segments of miniature roses that have been sterilized in advance as explants, after decontamination, sterilization and disinfection, the explants are cut into stem segments containing 2 intact scales, and the stem segments are immersed in the culture medium, wherein KT The content is 0.2mg / L, the 6-BA content is 1mg / L, the NAA content is 0.01mg / L, the pH value is 5.6, 0.4% activated carbon is added, the culture temperature is adjusted to 26°C, the light is 1300Lux for 12h, and the stem is induced. Segmented axillary buds.

[0034] The axillary buds were cut off and immersed in the medium, where the KT content was 0.2 mg / L, the 6-BA content was 0.1 mg / L, the NAA content was 0.1 mg / L, the pH value was 6, and the culture temperature was adjusted to 28 ° C, 1300 Lux Under the intensity of light for 12h, until the axillary buds grow to 2-3cm, normal seedlings are formed, and the seedlings are immersed in the rooting medium, wherein the...

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PUM

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Abstract

The invention discloses a miniature Chinese rose tissue culture breeding method. The miniature Chinese rose tissue culture breeding method is characterized by carefully selecting stem section explants and formula of an optimized culture medium, and combining technologies such as illumination to obtain a miniature Chinese rose with high survival rate. The miniature Chinese rose tissue culture breeding method specifically comprises the following steps: firstly taking stem sections with complete scale leaves selected from strong miniature Chinese roses as explants, then immersing the explants in the culture medium, irradiating by sunlight, generating axillary buds, taking down the axillary buds and immersing the axillary buds into a root growing culture medium, combining the illumination, transferring seedlings into a potted tray after the axillary buds grow to seedlings which are 2-3cm long, cooperating with technologies of coating films, regularly irradiating and sterilizing to obtain a tissue culture miniature Chinese rose. The miniature Chinese rose prepared by the method is high in survival rate and high in growth coefficient, and is suitable for industrialized mass production.

Description

Technical field: [0001] The invention belongs to the technical field of plant cultivation methods, in particular to a propagation method of micro rose tissue culture. Background technique: [0002] Rose is one of the top ten famous flowers in my country, also known as Yueyuehong, Yueyuekai, periwinkle, Four Seasons Rose, etc. It is an evergreen or semi-evergreen shrub belonging to the Rosaceae family. Rose has the advantages of rich color and pattern, long flowering period, and strong adaptability. Therefore, there are many varieties of roses, which are divided into fresh-cut roses, miniature roses, tree roses, strong-flowered roses, shrub roses, and ground cover roses. With the progress of society, the development of science and technology and the improvement of people's appreciation level, there are more and more varieties and colors in the flower season, and double colors, skin colors, and striped twisted colors are also becoming more and more common. [0003] Miniature ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陶建平
Owner 启东欣荣旅游开发有限公司
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