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Method and product for indicating alkaline lipase type time and temperature

A technology of time temperature and lipase, applied in thermometers, devices for measuring time intervals, thermometers with physical/chemical changes, etc., can solve problems such as economic losses, poor quality, improper temperature control, etc., and achieve the effect of reducing costs

Inactive Publication Date: 2009-01-21
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among all external environmental factors, temperature is a highly unpredictable factor, which often fluctuates due to changes in the environment. Improper temperature control in the storage environment will cause food spoilage and cause economic losses.
Because the quality of the product is also related to the storage time, under certain temperature conditions, the longer the storage time, the worse the quality

Method used

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  • Method and product for indicating alkaline lipase type time and temperature
  • Method and product for indicating alkaline lipase type time and temperature
  • Method and product for indicating alkaline lipase type time and temperature

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] (1) Establishment of time-temperature indicating reaction system

[0036] 1 Materials and methods

[0037] 1.1 Materials and Instruments

[0038] Materials: alkaline lipase (Shenzhen Lvweikang Bioengineering Co., Ltd., LVK-F100), triacetin, tributyrin, triolein, sodium hydroxide, glycine, polyvinyl alcohol 124, Calcium chloride, thymol blue, bromocresol green, phenol red, thymolphthalein, phenolphthalein, neutral red, absolute ethanol, etc.

[0039] Instruments: dispersing machine (IKA-ULTEA-TURRAX T 25 basic); pH meter (pH211, HANNAinstrument); portable colorimeter (Minolta-CR200, Japan); water bath, etc.

[0040] 1.2 Test method

[0041] 1.2.1 Selection of substrates

[0042] Glyceryl trioleate, glyceryl tributyrate and glyceryl triacetate were mixed with 20 g·L -1 PVA is mixed according to 1:19 (V:V) for emulsification, and the emulsification coefficient is set at: 9500r·min -1 , 30s each time, 10 times in total. Reaction conditions: take 8mL emulsion and add ...

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Abstract

The invention discloses an alkali lipase type time and temperature indicating process and a product, belonging to the technical field of farm product storage and transportation. The reaction system comprises using 0.1mL alkali lipase solution with 1gL-1 concentration, 0.5mL reaction substrate glyceryl triacetate, PVA9.5mL emulsifier with 20gL-1 concentration, 0.7mL CaC12 solution with 1molL-1 concentration, 39mL Gly-NaOH buffer solution with 0.05molL-1 concentration and 10.6pH, and 0.2mL phenol red-thymolphthalein mixing solution as the indicator of the reaction, deciding the relative relation with the time and temperature accumulating effect according to the reaction system color parameters values L*, a* and b* detected by CIE in the colourometry after starting the reaction. The alkali lipase type time and temperature indicating process of the invention can accurately indicate the time and temperature accumulating effect of objects in the process of production, storage, transportation and marketing.

Description

1. Technical field [0001] The invention relates to an alkaline lipase-type time and temperature indicating method and product, and belongs to the technical field of storage and transportation of agricultural products. It can be used to indicate the cumulative effect of time and temperature during the production, storage, transportation and sales of items, and to a certain extent, it can indicate the quality of the product. 2. Background technology [0002] The frequent occurrence of food quality and safety issues has aroused widespread concern. In addition to being affected by internal factors such as its own composition, food quality is also affected by external environmental conditions such as atmospheric pressure, mechanical pressure, light, and temperature, and quality and safety problems often occur during food production, storage, transportation, or shelf life, so control it well The external environment is a very important link. Among all external environmental fact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01K11/16G04F13/02
Inventor 徐幸莲周光宏宁鹏张楠
Owner NANJING AGRICULTURAL UNIVERSITY
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