Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Methods for identification of merle gene

一种基因、陨石的技术，应用在狗的遗传检验领域，能够解决陨石色基因没有被确定等问题

Inactive Publication Date: 2009-01-28
MERLOGEN LLC
View PDF22 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although there has clearly been a long-felt need for genetic testing methods that would allow the identification of animals with the merle and "cryptic" merle genotypes, the genes responsible for merle have so far not been identified

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods for identification of merle gene
  • Methods for identification of merle gene
  • Methods for identification of merle gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Embodiment 1: the screening of merle gene

[0117] Materials and methods

[0118] Sample Collection DNA samples were obtained from previous studies conducted at the Canine Genetics Laboratory at Texas A&M University, as well as through donations from participating owners and breeders. Whole blood or buccal cells were collected from all dogs, and genomic DNA was isolated using the Puregene DNA Isolation Kit (Gentra Systems, Minneapolis, MN, USA).

[0119] Genotyping Fluorescently labeled primers were synthesized and multiplex PCR was performed for the MSS-2 marker as described in Clark et al. (2004). PCR products were resolved using an ABI 3730x1 DNA Analyzer (PE Biosystems) with internal size standards (GeneScan 500LIZ, PE Biosystems, Foster City, CA, USA). use Software v3.5 (PE Biosystems) determined genotypes.

[0120] Linkage Analysis 41 Shetland Sheepdogs were used for LD analysis of all genotyped MSS-2 markers. For each marker, the allele more commonly asso...

Embodiment 2

[0132] Example 2: Determination of the variety specificity of the merle gene

[0133] To determine whether mutations in SILV caused merle patterns in the Shetland Sheepdog population were breed-specific, a pair representing six other breeds (collie, Australian shepherd, dachshund, Welsh corgi , Border Collie and Great Dane) insertion sequences from merle and non-merle dogs were analyzed. Merle dogs from all other breeds were heterozygous for this insertion (Figure 4).

[0134] Eleven brindle Great Danes were also analyzed: 9 were only heterozygous and 2 were only homozygous for the insertion. Sequence analysis showed that dogs from the above breeds had the same SINE insertion sequence in intron 10. An additional 29 dogs representing 26 non-merle breeds, which did not have the insertion, were analyzed.

[0135] Results overview

[0136] Insertion sequences were analyzed for 60 Shetland Sheepdogs used for linkage studies that segregated completely with the merle phenotype....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Animals with mutations in SILV present with the merle coat color pattern phenotype. Methods for the identification of animals that harbor a mutation in the SILV gene are described. Mutations in the SILV gene can be identified from any biological sample such as a cell or tissue that contains genomic DNA. A microsatellite marker identified using linkage disequilibrium mapping that segregates with merle is also described.

Description

Background of the invention [0001] The present invention is in the field of genetic testing of dogs in general, and in particular genetic testing of the genes responsible for the merle coat color. [0002] Merle is a dog coat pattern characterized by stripes of watered down color mixed with normal black (Fig. 1B). It is the standard coat color for several breeds recognized by the American Kennel Club, including the Shetland Sheepdog, Collie, Border Collie, Australian Shepherd (Australian Shepherd), Welsh Corgi (Cardigan WelshCorgi) and Dachshund (Dachshund). The merle phenotype of the Dachshund is also known as the brindle. Although merle is not an acceptable color in the Great Dane, the interaction of merle sites (M) and isolated harlequin sites (H) can produce the desired harlequin pattern (O'Sullivan, N., & Robinson, R. (1989) Genetica 78, 215-218). In addition, many other kennel society-accepted breeds (such as the Catahoula Leopard Dog, Norwegian Hound, and Pyrenean S...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q2600/156C12Q2600/16C12Q1/6883C12Q2600/124
Inventor 基思·E·墨菲利·安妮·克拉克杰奎琳·瓦尔克里斯汀·里斯
Owner MERLOGEN LLC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com