Method for producing triptolide and alkaloids by tripterygium wilfordii cell culture method
A technology of triptolide alkaloids and triptolide, applied in the field of triptolide and alkaloids produced by triptolide cell culture method, to achieve short production cycle and high efficiency
Inactive Publication Date: 2010-09-08
馥稷生物科技发展(上海)有限公司
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There is no report on the production of triptolide and total alkaloids by the two-step culture method of tripterygium wilfordii suspension cells
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The present invention discloses a method which utilizes the Tripterygium wilfordii cell culturing method to produce triptolide and Tripterygium wilfordii alkaloid. The method utilizes adventitious roots formed by the annual stuck branches of Tripterygium wilfordii as raw material to conduct callus induction and subculture on explants, establish suspension cell lines and establish and screen out cell suspension systems which can suitably utilize the two-step cell culturing method to produce the triptolide and the alkaloid, and corresponding growth culture media and formulae of producing a triptolide culture medium and a Tripterygium wilfordii alkaloid culture medium thereof. The method, which is utilized to produce the triptolide and total alkaloids from Tripterygium wilfordii, is characterized by short production cycle, high efficiency, environment-friendliness, etc. and helps to protect the natural Tripterygium wilfordii resource.
Description
Technical field The present invention relates to a method for producing triptolide and alkaloids, in particular to a method for producing triptolide and alkaloids by a tripterygium wilfordii cell culture method, the tripterygium wilfordii suspension culture cells produced by the method and the culture solution contained Triptolide and total alkaloids are relatively high, with short production cycle, high efficiency, and no pollution to the environment, and are beneficial to the protection of tripterygium wilfordii natural resources. Background technique Tripterygium (Tripterygium wilfordii Hook.f.) is a plant of the genus Tripterygium of the Euonymus family. It has been used in medicine and control of various pests long ago in our country, and is one of the famous insecticidal plants. Folks are mostly used to control vegetable pests, so it is called "vegetable medicine". It is effective against various pests. Traditional Chinese medicine can be used to treat swelling, edema, ...
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IPC IPC(8): C12N5/04C12P17/18
Inventor 李琰冯俊涛马志卿王智辉王永宏张兴
Owner 馥稷生物科技发展(上海)有限公司