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A kind of Xanthomonas polysaccharide culture medium and its preparation and application method

A technology of Xanthomonas polysaccharide and culture medium, which is applied in the field of preparation of Xanthomonas polysaccharide culture medium, can solve the problems such as no public records have been found, and achieve the effects of reducing production costs, reducing pollution, and realizing technological innovation

Inactive Publication Date: 2011-12-28
金翼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the above two topics has not found any public records

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Under normal temperature conditions, take 2L of potato starch wastewater, filter through sand filter, remove the solids such as potato residue, and obtain the supernatant for use. Dilute the supernatant with tap water and adjust the chemical oxygen demand to between 8000-10000. Take 1L of the above liquid, add NaOH or KOH to adjust its pH value to 4.0-6.0. Add 3% amylase by weight unit, stir evenly, and carry out hydrolysis at 45-55°C, and the hydrolysis time is 72 hours to obtain a reducing sugar solution with a concentration of 60-80 g / L. Add 2% weight unit of active medical stone particles (particle size 2-10mm) to the reducing sugar solution, soak for 8 hours at room temperature to decolorize and precipitate minerals K, Ca, Na, P, Se, etc., and get clarified after suction filtration After adding 2% yeast extract to the supernatant of the same, the Xanthomonas polysaccharide medium is obtained after stirring. Inoculate Xanthomonas campestris into the culture medium,...

Embodiment 2

[0024] Under normal temperature conditions, take 2L of potato starch wastewater, filter through sand filter, remove the solids such as potato residue, and obtain the supernatant for use. Dilute the supernatant with tap water and adjust the chemical oxygen demand to between 8000-10000. Take 1L of the above liquid, add NaOH or KOH to adjust its pH value to 4.0-6.0. Add 0.5% amylase by weight unit, stir evenly, and carry out hydrolysis at 45-55°C. The hydrolysis time is 36 hours to obtain a reducing sugar hydrolyzate with a concentration of 60-80 g / l. Add 0.5% weight unit of active medical stone particles (particle size 2-10mm) to the reducing sugar solution, soak for 12 hours at room temperature for decolorization, and precipitate minerals K, Ca, Na, P, Se, etc., after suction filtration A clear and transparent supernatant is obtained, and 1% by weight of yeast extract is added, and the xanthomonas polysaccharide culture medium is obtained after uniform stirring. Inject Xanthom...

Embodiment 3

[0026] Under normal temperature conditions, take 2L of corn starch wastewater, filter through sand filter, remove solids, and obtain supernatant for use. Dilute the supernatant with tap water and adjust the chemical oxygen demand to between 8000-10000. Take 1L of the above liquid, add NaOH or KOH to adjust its pH value to 4.0-6.0. Add 3% amylase according to the weight unit, stir evenly, and carry out hydrolysis at 45-55°C, and the hydrolysis time is 72 hours to obtain a reducing sugar solution with a reducing sugar of 60-80 g / l. Add 2% weight unit of active medical stone particles (particle size 2-10mm) to the reducing sugar solution, soak for 8 hours at room temperature to decolorize, and precipitate minerals K, Ca, Na, P, Se, etc., after suction filtration A clear and transparent supernatant was obtained, and 2% yeast extract was added, and the Xanthomonas polysaccharide medium was obtained after stirring. Inoculate Xanthomonas campestris into the culture medium, the inocu...

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Abstract

The invention discloses a Xanthomonas amylose culture medium, a method for preparing the culture medium and the application of the culture medium in the production of Xanthomonas amylose; the raw materials used for preparing the culture medium comprises starch waste water, amylase, active medical stone granule and yeast extract; the preparation method comprises the steps of wastewater disposal, pH value control, zymohydrolysis and saccharification, as well as depigmentation and adding nutrientsubstance; the method has the advantages that the starch waste water is transformed into the culture medium for producing the Xanthomonas amylase, thus greatly reducing the production cost and realizing the technological innovation of the industry of the Xanthomonas amylose; meanwhile, the method can turn harm into good and change waste into valuable, so as to reduce the pollution of organic wastewater of starch for the environment and develop circular economy in 'second panning' of the starch waste water.

Description

Technical field [0001] The present invention relates to a culture medium for preparing Xanthomonas polysaccharides. The present invention also relates to a preparation method of the culture medium. The present invention also relates to an application of the culture medium in the production of Xanthomonas polysaccharides. Background technique [0002] Xanthan gum, also known as Xanthan gum, is a monospore polysaccharide produced by the fermentation of Pseudoxanthomonas. This bacterial extracellular polysaccharide has an ultra-high molecular weight, which is biocompatible and biodegradable. Since its inception in the 1950s, this product has been widely used in more than 20 fields such as food, petroleum, and environmental protection. It is currently the world's largest-scale microbial polysaccharide with expanding uses. [0003] The production scale of Xanthomonas polysaccharides needs to be continuously developed and expanded. Of course, we must also follow several basic principles...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C02F3/02C02F103/26C12R1/64
CPCY02W10/10
Inventor 金翼张艳红富昱金夕梦高诗语
Owner 金翼
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