Novel triterpenoid schisanlactone H and extracting and separating method thereof
A technology for triterpenoids and pentalactones, applied in the field of phytochemistry, can solve the problems of no patents or literature reports on new triterpenoids, and achieve the effects of novel structure, good curative effect, and simple extraction and separation method.
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[0019] Take Schisandra sphenanthera fruit (dry weight 8kg) as raw material, according to the mass / volume ratio of Schisandra sphenanthera: acetone aqueous solution = 1: 3, extract three times with 70% acetone aqueous solution at room temperature, each time for 24 hour, the acetone consumption is 25L * 3, combine three extracts, concentrate under reduced pressure to no acetone smell; press extract: ethyl acetate=1: 1 volume ratio, extract is carried out 3 times with ethyl acetate, recover Obtain 145.9g of extract afterward; After silica gel column chromatography on the extract, use chloroform: acetone respectively: 1: 0, 9: 1, 8:: 2, 7: 3, 6: 4, 5:: 5, 0 : 1 volume ratio eluent, seven gradient elutions were carried out to obtain eight polar sections of A-H respectively, and section B (that is, the part eluted with chloroform: acetone = 9: 1) was successively washed with petroleum ether: acetone = 30 : 1 volume ratio eluent, chloroform: acetone = 50: 1 volume ratio eluent for si...
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