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Tibetan medicine Duyiwei total flavone extract and extraction method and use thereof

A technology for total flavonoids and extracts, applied in the field of medicine, can solve the problems of outdated preparation process, low content of active ingredients, large daily dosage, etc., and achieve the effects of improving transfer rate, improving utilization rate and saving production cost.

Active Publication Date: 2009-03-11
SHENYANG PHARMA UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation method of the current commercially available unique preparations is outdated, and they are all preparations prepared from the crude extract obtained after simple extraction of the unique medicinal material. The content of active ingredients is low and the daily dose is large.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take 2kg of unique medicinal material, pulverize, pass through a 20-mesh sieve, add 20000ml of ethanol solution with a volume fraction of 5% prepared by calcium hydroxide solution with a pH value of 9, reflux extraction for 1 hour, filter and save the filtrate, repeat the operation 4 times , combined the two filtrates, and concentrated the filtrate. Take the above concentrate, heat and dissolve with 50% ethanol, let it cool, filter to remove insoluble impurities, and spin the filtrate to remove ethanol. Take the above-mentioned rotary distilled liquid, dissolve it in water, extract four times with petroleum ether, and keep the water layer. The macroporous resin column that has been treated on the mother liquor (soaked in 95% ethanol for 12 hours, then eluted for 2-4 volumes with 95% ethanol, and then eluted with distilled water until the eluent has no alcohol smell), the flow rate is 1ml / min. Elute with a large amount of distilled water until the eluate is colorless,...

Embodiment 2

[0017] Take 2kg of unique medicinal material, pulverize, pass through a 20-mesh sieve, add 20000ml of ethanol solution with a volume fraction of 10% prepared by calcium hydroxide solution with a pH value of 8, reflux extraction for 1 hour, filter and save the filtrate, repeat the operation 4 times , combined four filtrates, and the filtrate was concentrated. Take the above concentrate, dissolve it by heating with 60% ethanol, let it cool, filter to remove insoluble impurities, and evaporate the filtrate to remove ethanol. Take the above-mentioned rotary distilled liquid, dissolve it in water, extract twice with petroleum ether, and keep the water layer. The macroporous resin column that has been treated on the mother liquor (soaked in 95% ethanol for 12 hours, then eluted for 2-4 volumes with 95% ethanol, and then eluted with distilled water until the eluent has no alcohol smell), the flow rate is 1ml / min. Elute with a large amount of distilled water until the eluate is col...

Embodiment 3

[0019] Take 2kg of unique medicinal material, pulverize, pass through a 20-mesh sieve, add 20000ml of ethanol solution with a volume fraction of 30% prepared by ammonia solution with a pH value of 8, reflux for extraction for 1 hour, filter and save the filtrate, repeat the operation 4 times, and combine Four times the filtrate was concentrated. Take the above-mentioned concentrate, dissolve it by heating with 70% ethanol, let it cool, filter to remove insoluble impurities, and evaporate the filtrate to remove ethanol. Take the above-mentioned rotary steamed liquid, dissolve it in water (the content of flavonoids is about 5mg·ml -1 ), extracted three times with petroleum ether, and retained the aqueous layer. The macroporous resin column that has been treated on the mother liquor (soaked in 95% ethanol for 12 hours, then eluted for 2-4 volumes with 95% ethanol, and then eluted with distilled water until the eluent has no alcohol smell), the flow rate is 1ml / min. Elute with...

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PUM

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Abstract

The invention belongs to the technical field of medicine, and provides a Tibetan pharmaceutical, lamiophlomis rotata total flavone extractive as well as an extracting method and the application thereof. The method uses Tibetan pharmaceutical lamiophlomis rotata as raw material, uses ethanol prepared by alkalescence solution with different pH values as solvent, and adopts a backflow method for extracting. After two steps of decontamination and the separation and enrichment of macropore resin, the total flavone extractive with the purity being 50 to 95 percent can be obtained from extracting solution. In the process of separation and enrichment, after the two steps of decontamination and the separation and enrichment of macropore resin, a sample with high purity can be obtained and the purity of the total flavone in the sample can be 95 percent at most, wherein, the percentage is higher than the number reported in documents. The invention also provides the application of the total flavone as pain killer medicine and stanch medicine. The paper optimizes and improves the extracting method so as to increase the transfer rate of effective ingredient, improve the utilization rate of medicinal materials and save a great amount of cost effectively, thus having high economical benefit.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to an extract of total flavonoids from unique Tibetan medicine and its extraction method and application. Background technique [0002] Duyiwei [Lamiophlomis rotata (Benthl) kudo] is one of the commonly used herbal medicines in Tibetan, Mongolian, Naxi and other ethnic groups in my country. It is mainly used for incision pain and bleeding after various surgical operations, traumatic fractures, sprains of muscles and bones, rheumatic arthralgia and other diseases. [0003] So far, the known chemical components in Duyiwei mainly include flavonoids, iridoids, and triterpene saponins, etc. according to literature reports. Studies have found that the total flavonoids contained in it have obvious analgesic and hemostatic effects. Duyiwei preparations include Duyiwei capsules, Duyiwei tablets, Duyiwei soft capsules, etc. The preparation process of the present commercially available unique ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53A61P7/04A61P29/00
Inventor 王淑君周国勤邱宁杨瑞
Owner SHENYANG PHARMA UNIVERSITY
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