Method for producing valerian iridoids
The technology of an iridoid and a production method is applied in the production field of valerian iridoid, can solve the problems of long total output period and low valerin product purity, and achieves low production cost and improved product purity. and quality, the effect of easy operation of the production process
Inactive Publication Date: 2010-11-10
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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Problems solved by technology
The above several methods are to concentrate and dry the extraction solvent, so the purity of the valerianin product obtained is not high, and the total output period is long
Method used
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The invention discloses a production method of valerian iridoid, which comprises the following steps: (1) induction cultivation and treatment of valerian callus: the explant of the valerian is inoculated to an MS solid medium containing corresponding plant growth regulatory substances for culture, successive transfer culture is carried out once every 15 days to 30 days, and the valerian callus containing more iridoid is selected; (2) cell suspension culture: the valerian callus is transferred to the liquid medium for gradual expansion culture and the valerian cells by expansion culture are inoculated to the liquid medium in a bioreactor for large-scale valerian cell suspension culture so as to obtain valerian cells; and (3) the iridoid is obtained by the extraction and separation of the valerian cells. The production method of the valerian iridoid resolves the problem that the production of valerian iridoid is short of raw materials, has high product purity, easy-control technical process and relatively low cost.
Description
Valerian iridoid production method technical field The invention relates to a production method of valerian iridoids. Background technique Valerian is a perennial herbaceous plant belonging to the genus Valeriana L. There are mainly four kinds of resources in our country: Valeriana officinalis L., Valeriana officinalis L.var. latifolia Miq., Valeriana officinalis L. var. West is mainly distributed in the south-central, southwest, northwest, and northeast mountainous and hilly areas. Valerian root mainly contains iridoids, sesquiterpenes, alkaloids, lignans, flavonoids and other bioactive components, which have significant antispasmodic effects on smooth muscle, increase coronary flow, antiarrhythmia, anti Tumor, sedative and soothe the nerves, etc., is an excellent natural drug resource. Valeriana currently used in my country is basically in the wild state, artificial planting has just begun, and the iridoid content in wild valerian can only reach a higher level in plan...
Claims
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IPC IPC(8): C12N5/04C12P17/06
Inventor 黎继烈李忠海方勤敏陈建华钟海雁彭映辉鲁海波王卫张慧
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY