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New nucleic acid molecules and collections thereof, their application and identification

A technology of nucleic acid molecules and aggregates, which is applied in the field of nucleic acid molecules and their aggregates, and can solve problems that have not been fully elucidated

Inactive Publication Date: 2009-04-01
KONINK NEDERLANDSE AKADE VAN WETENSCHAPPEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Another consequence, translational repression, is also not fully elucidated

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  • New nucleic acid molecules and collections thereof, their application and identification
  • New nucleic acid molecules and collections thereof, their application and identification
  • New nucleic acid molecules and collections thereof, their application and identification

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Embodiment Construction

[0140] Materials and Methods

[0141] Sequencing and analysis of miRNA regions in primates

[0142] Use primer3 software ( http: / / primers.niob.knaw.nl ) designed nested primer sets for PCR amplification of ~700bp regions of 144 known miRNA genes. The choice of primers was based entirely on the human sequence. Genomic DNA from 10 primate species (NIA Senescent Cell Bank DNA Panel PRP00001) (NIA Senescent Cell Bank DNA Panel PRP00001) was purchased from the Coriell Cell Bank (Camden, NJ). All PCR reactions were performed according to the manufacturer's conditions and overall cycle conditions (60 seconds at 94°C, followed by 30 cycles of 94°C for 20 seconds, 58°C for 20 seconds, and 72°C for 60 seconds) in a total volume of 10 μl with 0.5 units of Taq polymerase (Invitrogen, Carlsbad CA). PCR products were sequenced from both ends using an ABI3700 capillary sequencer (Applied Biosystems, Foster City CA). Sequences were quality trimmed and assembled with phred / phrap softwa...

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Abstract

The invention provides a method for characterising a sample comprising nucleic acid derived from a cell. The method comprises determining whether a sample comprises at least a minimal sequence of at least one new microRNA (miRNA) according to the invention or a mammalian ortholog thereof and characterizing the sample on the basis of the presence or absence of the miRNA. The invention further provides new nucleic acid molecules and collections thereof and their use in therapeutic and diagnostic applications. The invention furthermore provides a method for identifying a miRNA molecule or a precursor molecule thereof.

Description

technical field [0001] The present invention relates to nucleic acid molecules and assemblies thereof. The invention further relates to the use of nucleic acid molecules in therapeutic and diagnostic applications. The present invention also relates to methods for identifying miRNA molecules or precursor molecules thereof. Background technique [0002] MicroRNAs (miRNAs) are non-coding RNAs that regulate the expression of genes at the post-transcriptional level (reviewed in Bartel, 2004). However, it has only recently been discovered that microRNAs play critical roles in a variety of biological processes, including cell fate determination, cell death, proliferation, and fat storage (Brennecke, 2003, Poy et al., 2004, reviewed in Ambros, 2004 ). About 200 different miRNAs have been described for mouse and human (Griffiths-Jones, 2004). The molecular prerequisites and mechanisms by which miRNAs regulate gene expression have been elucidated (Bartel, 2004), but their respecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q2600/178C12Q2600/158C12Q1/6883A61P35/00C12N15/113C12N2310/141
Inventor 罗纳德·汉斯·安东·普拉斯特克尤金·别列济科夫埃德温·彼得·约翰·赫拉德·库佩恩
Owner KONINK NEDERLANDSE AKADE VAN WETENSCHAPPEN