In vitro application and detection method of peptide for regulating and controlling milk cattle rumen fermentation
A detection method and rumen technology, which are applied in the field of in vitro technology for regulating rumen fermentation of dairy cows, can solve problems such as unclear mechanism of action, and achieve the effects of rapid method, high repeatability, and improved quality of dairy products
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0054] 1. Example 1: Effect of different concentrations of peptides on artificial rumen fermentation parameters
[0055] 1. Test materials
[0056] (1) Artificial rumen simulator
[0057] A medical syringe with an inner diameter of 32mm, a length of 200mm, and a graduated volume of 100ml is used as the fermentor, and a water bath shaker that can accurately control the temperature (±0.5°C) is used as the temperature control device.
[0058] (2) Preparation of artificial rumen buffer (Menke solution)
[0059] The preparation of the buffer was carried out according to the method of Menke et al. (1979). After obtaining the rumen fluid, add the mixed culture solution: 400ml distilled water+0.1ml trace element solution A+200ml buffer solution B+200ml macro element solution C+1ml resazurin solution+40ml reducing agent solution. And mix well, heat to 39℃ while using CO 2 saturation.
[0060] Trace element solution (A): CaCl 2 ·2H 2 O-13.2g, MnCl 2 ·4H 2 O-10.0g, CoCl 2 ·6H 2 O-1.0g, FeCl 3 ·6H...
Embodiment 2
[0117] 2. Example 2: The effect of different concentrations of peptides on artificial rumen enzyme activity
[0118] 1. Test materials
[0119] Same as above
[0120] 2. Experimental design
[0121] Same as above
[0122] The microbial amylase activity, protease activity and carboxymethyl cellulase activity in the fermentation broth in the fermentation tube were measured at each time point.
[0123] 3. Combination of sample determination process and detection method figure 2 .
[0124] According to the conditions and characteristics of rumen microbial fermentation, amylase, protease and carboxymethyl cellulase (using sodium carboxymethyl cellulose as the substrate, mainly endoglucosidase, endo-1,4-β- D-glucanase, referred to as EG) three indicators press figure 2 Carry out the determination of rumen microbial enzyme activity.
[0125] (1) Determination of amylase activity
[0126] The rumen fluid was collected, centrifuged, and the supernatant was taken and measured with an amylase kit...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com