Entomogenous fungi powder for controlling pine greedy scale and production method and use thereof

A technology of insect-growing fungi and pine-shaped round scales, which is applied in the field of microbial preparations, can solve the problems of unsatisfactory control effects and low parasitic rate of control, achieve significant epidemic potential, reduce control costs, and reduce environmental pollution.

Active Publication Date: 2009-05-06
JIANGXI TIANREN ECOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The above prevention and control measures have the disadvantages of unsatisfactory prevention and control effect or low parasitism rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1: the first batch of bacterial powder production

[0059] In October 2007, the monthly average temperature was 27°C, the minimum temperature was 14°C, and the maximum temperature was 36°C; the atmospheric humidity was 50%-70%, which coincided with the severe drought period in Guangxi. In the laboratory, adopt potato solid medium (in 1000ml water: peeled potato 200mg, chopped and boiled for 30min to get the filtrate, sucrose 10g, agar 20g) to inoculate Pestalotiopsis olivacea bacterial block (diameter is 5mm), Cultivate at 28°C for 5 days to make a first-class strain. Enter the production workshop and carry out secondary cultivation, wherein: in the peanut cake culture fluid (50g of peanut cake powder, flour 50g sucrose 10g, KH 2 PO 4 0.5g, MgSO 4 0.2g, 1000ml of tap water) into a 1000ml large triangular flask, insert 1 dish of the first-level cultured bacteria, and cultivate for 3 days; add the bacteria liquid from the expansion culture of 2 bottles of tr...

Embodiment 2

[0060] Embodiment 2: the second batch of bacterial powder production:

[0061] At the beginning of March 2008, the monthly average temperature was 19°C, the lowest temperature was 10°C, and the highest temperature was 26°C; the atmospheric humidity was 90%-95%, which coincided with the rainy season after the severe freezing in Guangxi. In the laboratory, adopt potato solid medium (in 1000ml water: peeled potato 200mg, chopped and boiled for 30min to get the filtrate, sucrose 10g, agar 20g) to inoculate Pestalotiopsis olivacea bacterial block (diameter is 5mm), Cultivate at 28°C for 5 days to make a first-class strain. Enter the production workshop and carry out secondary cultivation, wherein: in the peanut cake culture fluid (50g of peanut cake powder, 50g of flour, sucrose 10g, KH 2 PO 4 0.5g, MgSO 4 0.2g, 1000ml of tap water) into a 1000ml large triangular flask, insert 1 branch of the first-level cultured strain, and cultivate for 3 days; add the bacterial solution expan...

Embodiment 3

[0064] 1. Prevention and control of pine-shaped round scale in winter

[0065] On November 25, 2007, before the overwintering of Saccharomyces pine, the inter-forest control was carried out in the 5th forest shift of Baimeilin Station, Sanyu Branch, Bobai Forest Farm, Guangxi. Imported mist dusters were used to operate. In order to ensure the control effect, The control was carried out at 6 o'clock in the morning when the dew on the leaves was still dry, and 7.5kg of fungus powder of the fungus growing on Scale pine was sprayed per hectare, covering a control area of ​​13.33 hectares. On December 21, 2007, February 25, 2008, and May 8, 2008, the average death rate of Saccharomyces pine was 95.68%, 96.22%, and 80.18%. It indicated that the developed Pestalotiopsis olivacea fungus powder had a good control effect on the pine scale in winter.

[0066] 2. Prevention and control of pine-shaped round scale in spring

[0067] On March 11, 2008, during the hatching stage of Scale pi...

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Abstract

The invention discloses an entomogenous fungi pulvis for controlling pine greedy scale and a production method and usages thereof. The entomogenous fungi pulvis for controlling pine greedy scale is formed by adopting a strain of Pestalotiopsis olivacea to process a culture through primary solid strain culturing, secondary liquid culturing and third solid culturing. The production method thereof comprises the following steps of: 1) the primary solid strain culturing; 2) the secondary liquid culturing; 3) the third solid culturing; 4) naturally drying a semi-finished product in the shade until the water content rate is between 10 and 14 percent; 5) using a cracker to crack and requiring the grain diameters to be between 0.2 and 0.5cm; and 6) packaging. The entomogenous fungi pulvis for controlling pine greedy scale is mainly used for controlling pine greedy scale, and can also be used for controlling Oracella acuta and is suitable for both draught areas and humid areas.

Description

technical field [0001] The invention relates to a microbial preparation, in particular to an entomogenic fungal powder for preventing and controlling Saccharomyces pine, as well as its production method and application. Background technique [0002] The area of ​​pine forests dominated by Pinus massoniana in the south of the Yangtze River in my country is more than 33 million ha 2 , is one of the most important vegetation components in my country, and is also the most important raw material for my country's wood-based panels, papermaking, forestry chemicals and other forestry industries. Pine trees have important economic, social and ecological benefits in my country. However, this important forest resource is currently facing a serious threat from Saccharomyces pine. The insect was first discovered in Guangdong Province in 1982. The forest stands with serious damage can die in pieces within 3 to 5 years. When it occurs in large numbers, it can reduce the volume growth of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P7/00
Inventor 黄宝灵方丽英吕成群王缉健
Owner JIANGXI TIANREN ECOLOGY
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