Entomogenous fungi powder for controlling pine greedy scale and production method and use thereof
A technology of insect-growing fungi and pine-shaped round scales, which is applied in the field of microbial preparations, can solve the problems of unsatisfactory control effects and low parasitic rate of control, achieve significant epidemic potential, reduce control costs, and reduce environmental pollution.
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Embodiment 1
[0058] Embodiment 1: the first batch of bacterial powder production
[0059] In October 2007, the monthly average temperature was 27°C, the minimum temperature was 14°C, and the maximum temperature was 36°C; the atmospheric humidity was 50%-70%, which coincided with the severe drought period in Guangxi. In the laboratory, adopt potato solid medium (in 1000ml water: peeled potato 200mg, chopped and boiled for 30min to get the filtrate, sucrose 10g, agar 20g) to inoculate Pestalotiopsis olivacea bacterial block (diameter is 5mm), Cultivate at 28°C for 5 days to make a first-class strain. Enter the production workshop and carry out secondary cultivation, wherein: in the peanut cake culture fluid (50g of peanut cake powder, flour 50g sucrose 10g, KH 2 PO 4 0.5g, MgSO 4 0.2g, 1000ml of tap water) into a 1000ml large triangular flask, insert 1 dish of the first-level cultured bacteria, and cultivate for 3 days; add the bacteria liquid from the expansion culture of 2 bottles of tr...
Embodiment 2
[0060] Embodiment 2: the second batch of bacterial powder production:
[0061] At the beginning of March 2008, the monthly average temperature was 19°C, the lowest temperature was 10°C, and the highest temperature was 26°C; the atmospheric humidity was 90%-95%, which coincided with the rainy season after the severe freezing in Guangxi. In the laboratory, adopt potato solid medium (in 1000ml water: peeled potato 200mg, chopped and boiled for 30min to get the filtrate, sucrose 10g, agar 20g) to inoculate Pestalotiopsis olivacea bacterial block (diameter is 5mm), Cultivate at 28°C for 5 days to make a first-class strain. Enter the production workshop and carry out secondary cultivation, wherein: in the peanut cake culture fluid (50g of peanut cake powder, 50g of flour, sucrose 10g, KH 2 PO 4 0.5g, MgSO 4 0.2g, 1000ml of tap water) into a 1000ml large triangular flask, insert 1 branch of the first-level cultured strain, and cultivate for 3 days; add the bacterial solution expan...
Embodiment 3
[0064] 1. Prevention and control of pine-shaped round scale in winter
[0065] On November 25, 2007, before the overwintering of Saccharomyces pine, the inter-forest control was carried out in the 5th forest shift of Baimeilin Station, Sanyu Branch, Bobai Forest Farm, Guangxi. Imported mist dusters were used to operate. In order to ensure the control effect, The control was carried out at 6 o'clock in the morning when the dew on the leaves was still dry, and 7.5kg of fungus powder of the fungus growing on Scale pine was sprayed per hectare, covering a control area of 13.33 hectares. On December 21, 2007, February 25, 2008, and May 8, 2008, the average death rate of Saccharomyces pine was 95.68%, 96.22%, and 80.18%. It indicated that the developed Pestalotiopsis olivacea fungus powder had a good control effect on the pine scale in winter.
[0066] 2. Prevention and control of pine-shaped round scale in spring
[0067] On March 11, 2008, during the hatching stage of Scale pi...
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