Method for inducement of cynomorium songaricum succulent stem callus and special-purpose culture medium thereof

Abstract

The invention discloses a method for inducing a Cynomorium songaricum succulent stem callus and a special culture medium thereof. The method for inducing the Cynomorium songaricum succulent stem callus comprises the following steps: a vascular tissue of a Cynomorium songaricum succulent stem is grafted in the culture medium of the Cynomorium songaricum succulent stem callus, and is darkly cultured at a culture temperature of between 15 and 25 DEG C for 45 to 55 days to obtain the callus. The adoption of the culture medium and the method for inducing the callus can overcome the dependency of the growth of the Cynomorium songaricum succulent stem on a host so as to improve the incidence rate of the Cynomorium songaricum succulent stem callus; and the callus grows well, and the inductivity can be up to 67 percent. The method lays a foundation for utilizing a tissue culture method to produce a substitute of Cynomorium songaricum, and ensures that the substitute performs a large quantity of division and proliferation under controllable conditions to accumulate medicinal components of the Cynomorium songaricum, thereby providing an effective way for wild resource protection and resourcedevelopment of the Cynomorium songaricum.

Description

A method of inducing callus of Cynomorium succulent stem and its special medium technical field The invention relates to a method for inducing plant callus, in particular to a method for inducing callus of Cynomorium succulent stem and a special culture medium thereof. Background technique Cynomorium is the fleshy stem of the plant Cynomorium songaricum Rupr. of Cynomoriaceae (Cynomoriaceae), Cynomorium songaricum Rupr. Parasitic on the roots of plants of the genus Nitraria L. in the family Zygophyllaceae, Cynomorium is mainly produced in the semi-desert or desert areas of Inner Mongolia and Qinghai, and is an important traditional Chinese medicine and Mongolian medicinal plant. Cynomorium yang is warm and sweet. It is recorded in "Compendium of Materia Medica": Cynomorium yang can nourish yin and qi, benefit essence and blood, relieve stool, moisten dryness and nourish tendons, and treat flaccidity and weakness. Modern medical research shows that Cynomorium is rich in v...

AI Technical Summary

Problems solved by technology

At present, there are mainly difficulties in the following aspects in the tissue culture process of Cynomorium: (1) Cynomorium is a total parasitic plant, and the key to tissue culture is to break its dependence on the host. The requirements are stricter, and the types and proportions of hormones need to be screened extensively; (2) Cynomorium cynomorium is rich in phenolic compounds, and the browning phenomenon is extremely serious during the cultivation process, and secretions overflow, which seriously affects the formation of callus; (3) The growth period of Cynomorium is as long as 4 to 5 years, and it is usually unearthed from May to June every year. This biological characteristic limits the time for tissue culture.

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