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Novel uses of plasmodium circumsporozoite protein in resisting proliferation and migration of tumor

A circumsporozoite protein and Plasmodium technology, applied in the field of protein, can solve the problem of inhibition of tumor proliferation and migration by Plasmodium CSP, and achieve good clinical application prospects

Inactive Publication Date: 2009-07-15
ARMY MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there is no report on the inhibition of tumor proliferation and migration by Plasmodium CSP

Method used

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  • Novel uses of plasmodium circumsporozoite protein in resisting proliferation and migration of tumor
  • Novel uses of plasmodium circumsporozoite protein in resisting proliferation and migration of tumor
  • Novel uses of plasmodium circumsporozoite protein in resisting proliferation and migration of tumor

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Embodiment Construction

[0017] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0018] One, the preparation of the recombinant expression vector of CSP

[0019] 1. Isolation of Plasmodium sporozoites and extraction of total RNA

[0020] Get Plasmodium yoelii BY265 strain, infect Anopheles stephensi according to routine method, on the 15th day after infection, dissect the salivary gland of infected Anopheles stephensi, isolate sporozoite; Use Trizol reagent (Roche company) to extract the total RNA of sporozoite , operate according to the instructions of the reagent, measure ...

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Abstract

The invention discloses a new application of plasmodium circumsporozoite protein CSP for preparing medicaments for anti-tumor multiplication and migration. The invention successfully constructs a recombinant expression carrier pFLAG-CMV8-CSP of the CSP and transfects the recombinant expression carrier into a human liver cancer cell strain HepG2 and a colorectal cancer cell strain SW480 in a manner of liposome transfection. The research result shows that the CSP is mainly expressed in the cytoplasm of HepG2 cells, can restrain TNF-Alpha and LPS, stimulate the HepG2 cells and SW480 cells to activate NF-Kappa B and restrain the multiplication of the SW480 cells, and is further developed into a novel protein medicament for the anti-tumor multiplication and migration hopefully; and the recombinant expression carrier containing the gene of the CSP is hopefully further developed into the novel protein medicament for the anti-tumor multiplication and migration, therefore, the invention has favorable clinical application prospect.

Description

technical field [0001] The invention relates to a new application of a protein, in particular to a new application of the malaria parasite circumsporozoite protein for anti-tumor proliferation and migration. Background technique [0002] Studies have found that nuclear factor-κB (NF-κB) is a bridge between inflammation and tumor. Tumor necrosis factor-α (TNF-α) secreted by tumor-educated macrophages binds to TNF-α receptors on the surface of tumor cell membranes, activates NF-κB, and promotes tumor survival and proliferation. Toll-like receptor (TLR) agonists can also activate NF-κB by binding to TLR on the surface of tumor cell membrane, and promote tumor survival and proliferation. Therefore, NF-κB inhibitors are an important means of treating tumors. [0003] Circumsporozoite protein (CSP) is the main protein covering the surface of Plasmodium sporozoites. It can mediate sporozoite-specific Sexual adhesion to the liver cell membrane is one of the important proteins for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61K48/00A61P35/00A61P35/04
CPCY02A50/30
Inventor 徐文岳丁艳
Owner ARMY MEDICAL UNIV
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